磁微粒化学发光法与胶体金法检测HIV感染与免疫印迹法确证对照分析  

Comparative Analysis of Magnetic Particle Chemiluminescence Method and Colloidal Gold Method for Detecting HIV Infection and Western Blotting Confirmation

作　　者：唐耀敏 唐贵文 TANG Yaomin;TANG Guiwen(Department of Clinical Laboratory,the Second People's Hospital of Guiyang,Guiyang 550081,China;不详)

机构地区：[1]贵阳市第二人民医院检验科,贵州贵阳550081

出　　处：《中国医学创新》2025年第6期144-148,共5页Medical Innovation of China

摘　　要：目的:以免疫印迹法(Western blotting,WB)为参照,分析磁微粒化学发光法、胶体金法对人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染筛查的效能。方法:于2023年7月3日—2024年10月31日选取400例在贵阳市第二人民医院进行HIV联合筛查的病例。所有病例均在本院进行磁微粒化学发光法、胶体金法检测,由贵州省临床检验中心进行WB检测。以WB检测结果为金标准,分析微粒化学发光法、胶体金法检测结果及其与WB检测的一致性,并比较微粒化学发光法、胶体金法筛查HIV感染的效能。结果:WB检测结果显示,269例HIV感染,131例无HIV感染。磁微粒化学发光法检测与WB检测的Kappa值为0.927,一致性良好。胶体金法检测与WB检测的Kappa值为0.875,一致性良好。磁微粒化学发光法诊断HIV感染的敏感度、准确度及阴性预测值均高于胶体金法,差异均有统计学意义(P<0.05);两种方法诊断HIV感染的特异度、阳性预测值比较,差异均无统计学意义(P>0.05)。结论:微粒化学发光法筛查HIV感染与WB具有较高一致性,且诊断敏感度、准确度均优于胶体金法。Objective:To analyze the efficacy of magnetic particle chemiluminescence method and colloidal gold method in screening for human immunodeficiency virus(HIV)infection,and the Western blotting(WB)as a reference.Method:A total of 400 cases were selected for HIV joint screening at the Second People's Hospital of Guiyang from July 3,2023 to October 31,2024.All cases were detected by magnetic particle chemiluminescence method and colloidal gold method in our hospital,and WB was detected by Guizhou Provincial Center for Clinical Laboratory.Taking WB detection results as the gold standard,the results of magnetic particle chemiluminescence method and colloidal gold method and their consistency with WB detection were analyzed,and the efficacy of magnetic particle chemiluminescence method and colloidal gold method in screening HIV infection was compared.Result:The WB detection results showed that 269 cases were HIV infected and 131 cases were not HIV infected.The Kappa value of magnetic particle chemiluminescence method detection and WB detection was 0.927,and the consistency was good.The Kappa value of colloidal gold method detection and WB detection was 0.875,and the consistency was good.The sensitivity,accuracy and negative predictive value of magnetic particle chemiluminescence method in the diagnosis of HIV infection were higher than those of colloidal gold method,the differences were statistically significant(P<0.05).There were no significant differences in specificity and positive predictive value in the diagnosis of HIV infection between the two methods(P>0.05).Conclusion:The magnetic particle chemiluminescence method screening for HIV infection has high consistency with WB,and the diagnostic sensitivity and accuracy are better than those of colloidal gold method.

关 键 词：人类免疫缺陷病毒 免疫印迹法 磁微粒化学发光法 胶体金法 

分 类 号：R446.6[医药卫生—诊断学] R512.91[医药卫生—临床医学]