人参皂苷Rg3对雄性小鼠生精功能的保护作用  

Protective Effect of Ginsenoside Rg3 on Spermatogenic Function in Male Mice

作　　者：罗新燕 解尧棋 程炜桓 王洪艳[1] 曲莉[1] LUO Xinyan;XIE Yaoqi;CHENG Weihuan;WANG Hongyan;QU Li(School of Public Health,Beihua University,Jilin City,Jilin Province 132013,China)

机构地区：[1]北华大学公共卫生学院,吉林吉林132013

出　　处：《吉林医药学院学报》2025年第1期7-9,共3页Journal of Jilin Medical University

基　　金：吉林省中医药科技项目(2023068);大学生创新创业训练项目(202310201145)。

摘　　要：目的 研究人参皂苷Rg3对雄性小鼠生精功能的保护作用。方法 30只清洁级C57BL/6雄性小鼠随机分为3组:对照组、邻苯二甲酸二丁酯(Dibutyl phthalate,DBP)组(400 mg/kg)以及DBP+Rg3组(400 mg/kg DBP和20 mg/kg Rg3),给药35 d后处死小鼠,测定其睾丸质量、精子密度和睾酮T值;HE染色进行观察。结果 与对照组相比,DBP组小鼠睾丸质量减小(P<0.05);相较于DBP组,DBP+Rg3组的小鼠睾丸质量有所增加(P<0.05)。与对照组比较,DBP组精子密度减小(P<0.05);与DBP组相比,DBP+Rg3组精子密度明显增高(P<0.05)。HE染色结果:与对照组相比,DBP组组织形态发生明显改变,中央空泡增大;与DBP组相比,DBP+Rg3组细胞空泡减小。与对照组相比,DBP组血清睾酮T值水平显著降低(P<0.05);与DBP组比较,DBP+Rg3组血清中T值升高(P<0.05)。结论 人参皂苷Rg3对于雄性小鼠的生精功能具有一定的保护作用。Objective To study the protective effect of ginsenoside Rg3 on spermatogenic function in male mice.Methods Thirty clean grade C57BL/6 male mice were randomly divided into three groups:control group,DBP group(400 mg/kg),and DBP+Rg3 group(400 mg/kg DBP and 20mg/kg Rg3).After 35 days of administration,the mice were euthanized,measure the testicular quality,sperm density and testosterone T value.HE staining for observation.Results Compared with the control group,the testicular mass of mice in the DBP group decreased(P<0.05),while the testicular mass of mice in the DBP+Rg3 group increased(P<0.05);compared with the control group,the sperm density in the DBP group decreased(P<0.05),while compared with the DBP group,the sperm density in the DBP+Rg3 group significantly increased(P<0.05);according to the HE staining results,compared with the control group,the DBP group showed significant changes in tissue morphology,with an increase in central vacuoles;compared with the DBP group,the DBP+Rg3 group showed a decrease in cell vacuoles;compared with the control group,the testosterone T value level in the serum was significantly reduced(P<0.05).Compared with the DBP group,the T value in the serum of the DBP+Rg3 group was increased(P<0.05).Conclusion Ginsenoside Rg3 has a certain protective effect on the spermatogenic function of male mice.

关 键 词：人参皂苷RG3 DBP 生精功能 睾酮 

分 类 号：R965.1[医药卫生—药理学]