蒙古黄芪中总皂苷提取工艺研究  

Study on Extraction Process of Total Saponins from Mongolian Astragalus

作　　者：邹悦 范晓兰 孔力[3] 雷钧涛[4] ZOU Yue;FAN Xiaolan;KONG Li;LEI Juntao(Changchun University of Traditional Chinese Medicine,Changchun 130117;Suzhou Xiangcheng District Hospital of Traditional Chinese Medicine,Suzhou,Jiangsu Province 215000;Jilin Hospital of Traditional Chinese Medicine,Jilin City,Jilin Province 132012;Jilin Medical University,Jilin City,Jilin Province 132013,China)

机构地区：[1]长春中医药大学,长春130117 [2]苏州市相城区中医医院,江苏苏州215000 [3]吉林市中医院,吉林吉林132012 [4]吉林医药学院,吉林吉林132013

出　　处：《吉林医药学院学报》2025年第1期10-14,共5页Journal of Jilin Medical University

基　　金：吉林省教育厅科学研究项目(JJKH20240596KJ);2024年度吉林省中医药科技项目(2024306)。

摘　　要：目的研究蒙古黄芪中总皂苷的最佳提取工艺。方法采用乙醇回流提取,以蒙古黄芪总皂苷得率为指标,通过单因素结合Box-Behnken响应面法考察乙醇浓度、乙醇用量、提取时间和提取次数4种因素对蒙古黄芪总皂苷得率的影响,建立回归模型并对回归模型进行验证,确定黄芪最佳提取工艺。结果回归模型显著,拟合精度高;影响蒙古黄芪总皂苷回流提取的顺序为乙醇用量>乙醇浓度>提取次数>提取时间,乙醇浓度和乙醇用量具有显著性,70%乙醇浓度和25倍的乙醇用量时蒙古黄芪总皂苷得率较多,而提取次数和提取时间不具显著性。结论蒙古黄芪的最佳提取工艺条件:70%乙醇浓度加入25倍乙醇用量,提取3次,每次90 min。此工艺下提取的蒙古黄芪总皂苷得率为3.201%,响应估计值与实际值吻合度良好。Objective To study the optimal extraction process of total saponins from Mongolian Astragalus in Mongolia.Methods The ethanol reflux extraction was used to determine the optimal extraction process of Mongolian Astragalus using the total saponin yield of Mongolian Astragalus as an index,and the effects of four factors,namely,ethanol concentration,ethanol dosage,extraction time,and number of times of extraction,were investigated by a one-way combination of Box-Behnken response surface method,and the regression model was established and validated to determine the optimal extraction process of Mongolian Astragalus.Results The regression model was significant and the fitting accuracy was high;the order of influencing the reflux extraction of total saponins from Mongolian Astragalus was ethanol dosage > ethanol concentration > extraction times > extraction time,ethanol concentration and ethanol dosage were significant,and more total saponins from Mongolian Astragalus were obtained with 70% ethanol concentration and 25 times of ethanol dosage,while the number of extractions and the time of extraction were not significant.Conclusion The optimum extraction process conditions for Mongolian Astragalus were 70% ethanol concentration,3 times extraction times,25 times ethanol dosage and 90 min extraction time.The yield of total saponins of Mongolian Astragalus extracted under this process was 3.201%.The response estimates were in good agreement with the actual valuse.

关 键 词：蒙古黄芪 总皂苷 响应面法 提取工艺 

分 类 号：R285.5[医药卫生—中药学]