22份金线莲种质资源的耐热性评价与分析  

Evaluation and Analysis of Heat Resistance of 22 Anoectochilus roxburghii Germplasm Resources

作　　者：吴梅 孔向军 周小军 方莉 赵俊跃 马巧群 郑祎晨 包根玉 WU Mei;KONG Xiang-jun;ZHOU Xiao-jun;FANG Li;ZHAO Jun-yue;MA Qiao-qun;ZHENG Yi-chen;BAO Gen-yu(Jinhua Academy of Agricultural Sciences,Jinhua 321007,China;Zhejiang Jiankang Agricultural Technology Co.,Ltd.,Jinhua 321007,China;Shafan Baogenyu Family Farm of Wucheng District,Jinhua 321007,China)

机构地区：[1]金华市农业科学研究院,浙江金华321007 [2]浙江匠康农业科技有限公司,浙江金华321007 [3]金华市婺城区沙畈包根玉家庭农场,浙江金华321007

出　　处：《江西农业学报》2025年第2期36-43,共8页Acta Agriculturae Jiangxi

基　　金：浙江省农业新品种选育重大科技专项(2021C02074-4-3);浙江省金华市科技计划项目(2023-2-005)。

摘　　要：以22份不同来源的金线莲品种(系)设施组培苗作为供试材料,开展了日温38℃/夜温30℃的高温胁迫和日温25℃/夜温20℃的温度适应性恢复试验,并观测其热害指数、相对电导率,叶绿素、Pro、MDA、GSH含量,以及CAT、POD、APX酶活性,结果表明:(1)随着高温胁迫时间的延长,金线莲的相对电导率、Pro和MDA含量持续上升,而叶绿素和GSH含量、CAT、POD、APX酶活性则显著下降,且不同品种(系)之间存在明显的差异;(2)叶绿素降解率与热害指数呈极显著正相关(r=0.893),MDA含量与热害指数呈显著正相关(r=0.835),因此,叶绿素降解率和MDA含量可以作为金线莲耐热性筛选和评价的指标;(3)在22份金线莲品种(系)中,耐热性较强的品种(系)为K、H、X、J、S、G、T1、金康1号。The greenhouse seedlings of Anoectochilus roxburghii from 22 different provenances were used as experimental materials.Under the conditions of high temperature stress at 38℃and 30℃at night,and recovery at suitable temperature at 25℃and 20℃at night,the heat damage index,relative conductivity,chlorophyll,Pro,MDA,GSH,CAT,POD and APX were observed.The results showed that:(1)With the prolongation of high temperature stress time,the relative conductivity,Pro and MDA contents of Anoectochilus roxburghii continued to increase,while Chlorophyll and GSH contents,CAT,POD and APX enzyme activities decreased significantly,and there were obvious differences among varieties.(2)There was a very significant positive correlation between chlorophyll degradation rate and heat damage index(r=0.893),and there was a significant positive correlation between MDA and heat injury index(r=0.835).Therefore,chlorophyll degradation rate and MDA content could be used as reliable indexes for screening and evaluating heat resistance of Anoectochilus roxburghii.(3)Among 22 Anoectochilus roxburghii varieties,K,H,X,J,S,G,T1 and Jiangkang 1 had stronger heat resistance.

关 键 词：金线莲 耐热性 筛选 评价与分析 

分 类 号：S646.7[农业科学—蔬菜学]