海洋放线菌 Streptomyces sp.11695 次生代谢产物的研究  

Secondary metabolisms of marine-derived Streptomyces sp.11695

作　　者：颜祯阳 李晓 王远强 罗明和 YANG Zhen-yan;LI Xiao;WANG Yuan-qiang;LUO Ming-he(School of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing 400054,China)

机构地区：[1]重庆理工大学药学与生物工程学院,重庆400054

出　　处：《天然产物研究与开发》2025年第2期278-282,共5页Natural Product Research and Development

基　　金：重庆市科委面上项目(CSTB2022NSCQ-MSX0995);国家自然科学基金面上项目(32370083);重庆市教委项目(KJQN202301118)。

摘　　要：以抑菌活性为向导,研究海洋放线菌Streptomyces sp.11695的抑菌活性成分,并根据各个产物的理化性质鉴定其化学结构。采用色谱层析技术和抑菌活性为导向的分离方法,对从海洋放线菌Streptomyces sp.11695在AM3培养基的发酵产物进行分离,从中分离获得6个次级代谢产物,质谱(MS)和核磁共振波谱(NMR)数据分析,鉴定其分别为mucidone(1)、dixiamycin B(2)、xiamycin A(3)、xiamycin B(4)、nocapyrone A(5)、lobophorin CR1(6)。化合物1~6首次从菌株Streptomyces sp.11695中同时分离获得。抗菌活性结果显示,化合物2、3、4、6抗菌活性良好,其中化合物2对测试致病菌的最低抑菌浓度(minimum inhibitory concentration,MIC)为1.1~4.5μg/mL。本文通过改变培养条件的策略,成功从安莎菌素产生菌Streptomyces sp.11695中再次同时分离鉴定3种不同结构类型的次级代谢产物,进一步证实了菌株有合成多种次级代谢产物的能力。This study aims to investigate the antibacterial components of marine-derived actinomycete Streptomyces sp.11695 guided by antibacterial activity,and identify their structures on the basis of their physicochemical properties.Six compounds were isolated from the extracts of marine-derived Streptomyces sp.11695 by bioactivity guided isolation method,which is fermented in AM3 medium.These compounds are identified as mucidone(1),dixiamycin B(2),xiamycin A(3),xiamycin B(4),nocapyrone A(5),and lobophorin CR1(6),respectively,on the basis of their MS and NMR spectroscopic data.Compounds 1-6 was isolated from the strain of Streptomyces sp.11695 for the first time.Antibacterial activity results showed that compounds 2,3,4,6 showed good antimicrobial bioactivity,among them compound 2 showed the minimum inhibitory concentration(MIC)value of 1.1-4.5μg/mL.Compounds of three types were simultaneously isolated and identified from Streptomyces sp.11695 by changing fermentation medium,which further confirmed that this strain has the ability to synthesize multiple secondary metabolites.

关 键 词：xiamycin nocapyrone lobophorin 海洋放线菌 次级代谢产物 

分 类 号：R629.3[医药卫生—整形外科]