菊苣根多糖酶法辅助双水相提取工艺优化及其降脂活性研究  

Optimization of enzyme-assisted aqueous two-phase extraction process for chicory root polysaccharides and its hypolipidemic activity

作　　者：周敏[1] 韩青 李彩凤 ZHOU Min;HAN Qing;LI Cai-feng(Jiangsu Food&Pharmaceutical Science College,School of Pharmaceutical Engineering,Huai′an 223005,China)

机构地区：[1]江苏食品药品职业技术学院制药工程学院,淮安223005

出　　处：《天然产物研究与开发》2025年第2期283-292,共10页Natural Product Research and Development

基　　金：江苏省高等学校基础科学(自然科学)研究重大项目(23KJA350001)。

摘　　要：本研究旨在优化菊苣根多糖(chicory root polysaccharides,CRP)的酶法辅助双水相提取工艺,并评估其降脂活性。研究采用Box-Behnken设计与响应面法对提取工艺进行优化,考察纤维素酶添加量、果胶酶添加量、(NH 4)2SO 4质量分数和聚乙二醇(polyethylene glycol,PEG)质量分数对CRP得率的影响。结果表明,纤维素酶添加量、果胶酶添加量、(NH 4)2SO 4质量分数和PEG质量分数对多糖得率均有显著影响。优化后的最佳提取条件为:纤维素酶添加量2.00%,果胶酶添加量2.00%,(NH 4)2SO 4质量分数18.00%,PEG质量分数15.00%,在此条件下多糖得率可达32.78%。此外,本研究还发现CRP能够有效减少脂肪化HepG2细胞内脂质沉积,降低总胆固醇和甘油三酯含量,表明其具有良好的降脂活性。This study aims to optimize the enzyme-assisted aqueous two-phase extraction process of chicory root polysaccharides(CRP)and evaluate its lipid-lowering activity.The research employed the Box-Behnken design and response surface methodology to optimize the extraction process,examining the effects of the amounts of cellulase and pectinase added,the mass fraction of(NH 4)2SO 4,and the mass fraction of polyethylene glycol(PEG)on the yield of CRP.The results indicated that the amounts of cellulase,pectinase,(NH 4)2SO 4 mass fraction,and PEG mass fraction all significantly affected the yield of polysaccharides.The optimized extraction conditions were cellulase amount of 2.00%,pectinase amount of 2.00%,(NH 4)2SO 4 mass fraction of 18.00%,and PEG mass fraction of 15.00%,under which the yield of polysaccharides can reach 32.78%.Additionally,this study found that CRP can effectively reduce lipid deposition in fatty HepG2 cells,decrease total cholesterol and triglyceride levels,indicated their good lipid-lowering activity.

关 键 词：菊苣根多糖 酶法辅助双水相提取 响应面法 降脂活性 

分 类 号：R284.2[医药卫生—中药学] R285[医药卫生—中医学]