基于RGS14/MEK/ERK信号通路研究石菖蒲和 α -细辛醚促进疲劳运动大鼠学习记忆的作用机制  

Effect of Acorus tatarinowii Schott andα-asarone on learning and memory of rats with fatigue exercise based on RGS14/MEK/ERK signaling pathway

作　　者：廖忠鑫 谢石凤 朱梅菊[1] 王文莉[1] LIAO Zhong-xin;XIE Shi-feng;ZHU Mei-ju;WANG Wen-li(School of Sports Science,Jinggangshan University,Ji′an 343009,China)

机构地区：[1]井冈山大学体育学院,吉安343009

出　　处：《天然产物研究与开发》2025年第2期304-310,共7页Natural Product Research and Development

基　　金：江西省自然科学基金(20192BAB205092),江西省教育厅自然科学基金(GJJ2201646);国家自然科学基金(31660291);井冈山大学博士启动基金(JZB11043)。

摘　　要：研究石菖蒲和α-细辛醚抵抗大鼠疲劳运动后学习记忆下降的RGS14/MEK/ERK信号通路作用机制。80只SD大鼠按完全随机法分为:正常组(normal,Nor)、运动组(exercise,E)、运动+石菖蒲组(exercise+Acorus tatarinowii Schott,EAT)、运动+α-细辛醚组(exercise+α-asarone,EαA)、运动+石菖蒲+RGS14拮抗剂CCG-63802组(EATC)、运动+α-细辛醚+RGS14拮抗剂CCG-63802(EαAR)、运动+石菖蒲+MEK拮抗剂U0126组(EATU)、运动+α-细辛醚+MEK拮抗剂U0126组(EαAU),每组10只。开展水迷宫实验进行学习记忆检测;利用免疫组化、免疫印迹(Western blot)和荧光实时定量PCR(RT-PCR)等方法检测G蛋白信号调节蛋白14(regulator of G protein signaling 14,RGS14)、Raf-1、磷酸化丝裂原活化蛋白激酶激酶(phosphorylated mitogen-activated protein kinase kinase,p-MEK)和磷酸化细胞外信号调节激酶1/2(phosphorylated extracellular signal-regulated kinase 1/2,p-ERK1/2)表达水平。结果表明EAT和EαA组大鼠逃避潜伏期显著低于E组;穿越平台次数显著高于E组(P<0.01)。EATC和EαAR组大鼠逃避潜伏期最短,穿越平台次数最多(P<0.01)。EATU和EαAU大鼠逃避潜伏期最长,穿越平台次数最少(P<0.01)。EAT和EαA组RGS14蛋白和mRNA表达水平显著低于E组,Raf-1、p-MEK和p-ERK1/2表达水平则显著高于E组(P<0.01)。EATC和EαAR组RGS14蛋白和mRNA表达水平最低,显著低于其他各组,Raf-1、p-MEK和p-ERK1/2表达水平最高,显著高于其他各组(P<0.01或P<0.05)。EATU和EαAU组p-MEK、p-ERK1/2表达水平最低,显著低于其他各组(P<0.01)。以上实验结果表明石菖蒲和α-细辛醚能够基于对海马RGS14/MEK/ERK信号通路的调节,从而显著提高疲劳运动大鼠的学习记忆。This study aims to explore the effect of Acorus tatarinowii Schott andα-asarone on RGS14/MEK/ERK signal pathway in hippocampus of rats with fatigue exercise,and study its mechanism on improving learning and memory of rats with fatigue exercise.Eighty SD male rats were randomly divided into eight groups:control group(Nor),exercise group(E),exercise+α-asarone group(EαA),exercise+A.tatarinowii group(D),exercise+α-asarone+CCG-63802 group(EαAR),exercise+A.tatarinowii+CCG-63802 group(EATC),exercise+α-asarone+U0126 group(EαAU),exercise+A.tatarinowii+U0126 group(EATU).There were ten rats in each group.Water maze experiment was used to test learning and memory.The expression levels of regulator of G protein signaling 14(RGS14),Raf-1,phosphorylated mitogen-activated protein kinase kinase(p-MEK)and phosphorylated extracellular signal-regulated kinase 1/2(p-ERK1/2)were detected by the methods of immunohistochemistry,Western blot and RT-PCR.The results showed that the escape latency in group EAT and EαA was significantly lower than that in group E,and the number of platform crossings was significantly higher than that in group E(P<0.01).Group EATC and EαAR had the shortest escape latency and the most times of crossing the platform(P<0.01).Group EATU and EαAU had the longest escape latency and the least number of platform crossings(P<0.01).The expression levels of RGS14 protein and mRNA in group EAT and EαA were significantly lower than those in group E,while the protein expression levels of Raf-1,p-MEK and p-ERK1/2 were significantly higher in group EAT and EαA than those in group E(P<0.01).The expression levels of RGS14 protein and mRNA in EATC and EαAR groups were the lowest and significantly lower than those in the other groups,while the protein expression levels of Raf-1,p-MEK and p-ERK1/2 in group EATC and EαAR were the highest and significantly higher than those in the other groups(P<0.01 or P<0.05).The protein expression levels of p-MEK,p-ERK1/2 in group EATU and EαAU were the lowest and significantly lo

关 键 词：石菖蒲 Α-细辛醚 学习记忆 运动性疲劳 RGS14/MEK/ERK信号通路 

分 类 号：R34[医药卫生—基础医学]