Role and mechanism of Roux-en-Y gastric bypass in the treatment of diabetic urinary bladder hyperactivity by reducing TRPV1 and P2X3  

作　　者：Guang-Yong Li Shuai Ren Bin-Cheng Huang Jia-Jin Feng Qiang-Qiang Wang Qing-Jie Peng Hai-Fu Tian Le-Yi Yu Cun-Ling Ma Shu-Zhe Fan Xiao-Jiang Chen Mohammed Abdulkarem Al-Qaisi Rui He 

机构地区：[1]Department of Urology,General Hospital of Ningxia Medical University,Yinchuan 750000,Ningxia Hui Autonomous Region,China [2]Peking University First Hospital Ningxia Women's and Children's Hospital,Yinchuan 750000,Ningxia Hui Autonomous Region,China [3]Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education,Ningxia Medical University,Yinchuan 750000,Ningxia Hui Autonomous Region,China

出　　处：《World Journal of Diabetes》2025年第4期205-219,共15页世界糖尿病杂志(英文)

基　　金：Supported by National Natural Science Foundation of China,No.81860268 and No.82201000;Ningxia Natural Science Foundation,No.2021AAC02025;Ningxia Science and Technology Innovation Leading Talent Training Project;No.2020GKLRLX06 and No.2020GKLRLX11;Ningxia Medical University Research Project,No.XJKF240315;Ningxia Key Research and Development Project,No.2023BEG03021 and No.2021BEB04034.

摘　　要：BACKGROUND Diabetes mellitus(DM)is linked to an earlier onset and heightened severity of urinary complications,particularly bladder dysfunction,which profoundly impacts patient quality of life.Overactive bladder(OAB)is a common storage disorder of the lower urinary tract and is characterized by urgency,frequency,and nocturia.Several factors contribute to bladder dysfunction in diabetic individuals,including changes in urothelial signaling,detrusor morphology,and central nervous system regulation.The transient receptor potential vanilloid type 1 channel,expressed by bladder urothelial cells,is upregulated in OAB and plays a crucial role in ATP release during bladder filling.This ATP release subsequently activates purinergic receptor P2X3,further exacerbating OAB symptoms.AIM To clarify the mechanism of Roux-en-Y gastric bypass(RYGB)metabolic surgery to improve OAB in type 2 DM(T2DM).METHODS The model of T2DM was induced by feeding a high-fat diet to mice for 16 weeks.After 16 weeks,sham operation and RYGB operation were performed.The related indexes of glucose metabolism were also detected to evaluate the therapeutic effect,and the recovery degree of bladder function and micturition behavior of mice was assessed by urodynamics and micturition spot analysis.RESULTS Compared with the normal mice in the sham group,T2DM mice had increased urine spot count,uncontrolled urination behavior,shortened urination interval,and reduced bladder capacity.Immunohistochemistry and immunofluorescence costaining showed that Transient receptor potential vanilloid type 1(TRPV1)and purinergic receptor P2X3 were both expressed in mouse bladder epithelial layer,and they had the same localization.In the bladder of T2DM mice,the mRNA and protein expression of TRPV1 and P2X3 were significantly increased.The ATP content in urine of T2DM mice was significantly higher than that of the sham group.After RYGB operation,the glucose metabolism index of the RYGB group was significantly improved compared with the OAB group.Comparing the results of ur

关 键 词：Roux-en-Y gastric bypass Diabetes BLADDER Transient receptor potential vanilloid type 1 P2X3 

分 类 号：R587.1[医药卫生—内分泌]