复硝酚钠缓解二氯吡啶酸对油菜药害的研究  

Study on the alleviation of phytotoxicity of clopyralid by sodium nitrophenolate to oilseed rape

作　　者：王富豪 郭婧华 郝培培 程有普 Wang Fuhao;Guo Jinghua;Hao Peipei;Cheng Youpu(College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin 300392,China;Tianjin Lüheng Chemical Co.,Ltd.,Tianjin 300270,China)

机构地区：[1]天津农学院园艺园林学院,天津300392 [2]天津市绿亨化工有限公司,天津300270

出　　处：《天津农学院学报》2025年第1期19-24,共6页Journal of Tianjin Agricultural University

基　　金：国家自然科学基金(31572034);天津市企业科技特派员项目(19JCTPJC59000)。

摘　　要：为探究复硝酚钠(Sodium nitrophenolate)缓解二氯吡啶酸(Clopyralid)对油菜药害的作用机制,采用室内盆栽试验,油菜幼苗三叶一心时,一组喷施0(CK)、0.4、0.8(推荐剂量)、1.6、3.2、4.0 g/L二氯吡啶酸,另一组药后24 h叶面喷施9 mg/L复硝酚钠,比较两组油菜叶片的抗氧化酶活性、叶绿素及类胡萝卜素含量变化。结果表明:喷施二氯吡啶酸后,油菜幼苗的抗氧化酶(CAT、POD、SOD)活性、还原型谷胱甘肽(GSH)含量随质量浓度升高呈先升高后降低的变化趋势,二氯吡啶酸质量浓度为1.6g/L时,CAT活性最高,质量浓度为3.2g/L时,CAT活性升高幅度降低,叶绿素和类胡萝卜素含量与CK相比分别下降30.2%、6.0%,丙二醛(MDA)含量升高165.6%,质量浓度4.0g/L时,POD、SOD活性、GSH含量分别比CK低11.0%、26.0%、17.7%,MDA含量升高195.5%,叶绿素和类胡萝卜素含量分别下降62.9%、23.7%;喷施复硝酚钠后,与相同质量浓度的二氯吡啶酸处理相比,油菜叶片CAT、POD、SOD活性和GSH、叶绿素、类胡萝卜素含量均有明显升高,与缓解前相比MDA含量降低8.7%~44.5%,POD活性升高7.0%~11.8%,SOD活性升高10.1%~68.7%,GSH含量升高8.5%~19.6%,叶绿素含量升高7.0%~28.9%,类胡萝卜素含量升高7.1%~62.0%。综上可知,低质量浓度的二氯吡啶酸能够提高油菜叶片抗氧化酶活性和GSH含量,从而提高油菜的抗逆性,二氯吡啶酸质量浓度过高时,油菜叶片的MDA含量明显升高,抗氧化酶活性、GSH含量、叶绿素含量、类胡萝卜素含量受到明显抑制,喷施复硝酚钠后抑制作用得到不同程度的缓解,随二氯吡啶酸质量浓度升高缓解效果减弱,表明复硝酚钠可以在一定程度上减轻二氯吡啶酸对油菜的药害。In order to explore the mechanism of sodium nitrophenolate alleviating the phytotoxicity of clopyralid on rape,an indoor pot experiment was carried out.When the three leaves of rape seedlings were one heart,one group was sprayed with 0(CK),0.4,0.8(recommended dose),1.6,3.2,4.0 g/L clopyralid,and the other group was sprayed with 9 mg/L sodium nitrophenolate on the leaf surface 24 h after treatment.The changes of antioxidant enzyme activity,chlorophyll and carotenoid content in the leaves of the two groups were compared.The results showed that the activities of antioxidant enzymes(CAT,POD,SOD)and the content of reduced glutathione(GSH)in rape seedlings increased first and then decreased with the increase of mass concentration of clopyralid.When the mass concentration of clopyralid was 1.6 g/L,the activity of CAT was the highest.When the mass concentration was 3.2 g/L,the increase of CAT activity decreased,the contents of chlorophyll and carotenoid decreased by 30.2%and 6.0%respectively compared with CK,and the content of malondialdehyde(MDA)increased by 165.6%,when the mass concentration was 4.0 g/L.POD,SOD activity and GSH content were 11.0%,26.0%and 17.7%lower than CK,MDA content increased by 195.5%,chlorophyll and carotenoid content decreased by 62.9%and 23.7%,respectively.After spraying sodium nitrophenolate,the activities of CAT,POD,SOD and the contents of GSH,chlorophyll and carotenoid in rape leaves were significantly increased compared with those treated with the same concentration of clopyralid.Compared with those before remission,MDA content decreased by 8.7%-44.5%,POD activity increased by 7.0%-11.8%,SOD activity increased by 10.1%-68.7%,GSH content increased by 8.5%-19.6%,chlorophyll content increased by 7.0%-28.9%,and carotenoid content increased by 7.1%-62.0%.In summary,low mass concentration of clopyralid can increase the antioxidant enzyme activity and GSH content of rape leaves,thereby improving the stress resistance of rape.When the mass concentration of clopyralid is too high,the MDA content of r

关 键 词：二氯吡啶酸 复硝酚钠 油菜 缓解作用 

分 类 号：TQ450.1[化学工程—农药化工]