一株源自蛋鸡的沃氏葡萄球菌分离鉴定与耐药性分析  

Isolation, identification and drug resistance analysis of a strain of Staphylococcus warneri from laying hens

作　　者：冉恒东 吴定洲 王永贞 刘燕霏 杨建德 Ran Hengdong;Wu Dingzhou;Wang Yongzhen;Liu Yanfei;Yang Jiande(College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300392,China)

机构地区：[1]天津农学院动物科学与动物医学学院,天津300392

出　　处：《天津农学院学报》2025年第1期39-43,共5页Journal of Tianjin Agricultural University

基　　金：天津市应用基础及前沿技术研究计划项目(10JCYBJC09400)。

摘　　要：沃氏葡萄球菌是一种凝固酶阴性葡萄球菌,近年来由该病原菌引起的感染发病率逐渐升高,且呈现出多重耐药性。本研究对来自天津市某鸡场成年蛋鸡化脓性病灶进行细菌分离,并对分离到的菌株进行染色镜检、生化试验、药敏试验及16S rRNA基因测序鉴定,对测序结果进行同源性分析并构建系统发育树。结果表明:该分离菌染色镜检为革兰氏阳性球菌,生化和培养结果符合葡萄球菌的特性,16S rRNA基因序列分析与沃氏葡萄球菌同源性高达99.8%,确定分离菌为沃氏葡萄球菌。该分离菌对四环素、米诺环素、头孢曲松、头孢喹肟、诺氟沙星、环丙沙星、左氟沙星、克林霉素、万古霉素等敏感,对青霉素G、红霉素、克拉霉素耐药。本研究结果可为防控和治疗由沃氏葡萄球菌引起的感染提供参考依据。Staphylococcus warneri is a coagulase negative Staphylococcus.In recent years,the incidence rate of infection caused by this pathogen has gradually increased and showed multiple drug resistance.In this study,the bacteria were isolated from the purulent lesion of adult laying hens from a chicken farm in Tianjin,and the isolated strains were identified by staining microscopy,biochemical test,drug sensitivity test and 16S rRNA gene sequencing.The homology of the sequencing results was analyzed and a phylogenetic tree was constructed.The results showed that gram-positive cocci were detected by staining microscopy,and the biochemical and culture results were consistent with the characteristics of Staphylococcus.The 16S rRNA gene sequence analysis showed a high homology of 99.8%with the warning type Staphylococcus,and the isolated strain was determined to be Staphylococcus warneri.The isolated bacteria were sensitive to tetracycline,minocycline,ceftriaxone,cefquinoxime,norfloxacin,ciprofloxacin,levofloxacin,clindamycin and vancomycin,and resistant to penicillin G,erythromycin and clarithromycin.The results of this study can provide reference for the prevention,control and treatment of infection caused by Staphylococcus warneri.

关 键 词：沃氏葡萄球菌 生化试验 药敏试验 16S rRNA 系统发育树 耐药性 

分 类 号：S852.61[农业科学—基础兽医学]