结核分枝杆菌富集技术的研究进展  

Research advances of Mycobacterium tuberculosis enrichment technology

作　　者：黄咪孙 武娅宁 李桂莲[1] 刘海灿[1] Huang Misun;Wu Yaning;Li Guilian;Liu Haican(National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,Department of Tuberculosis Control,National Institute for Communicable Disease Controland Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]传染病溯源预警与智能决策全国重点实验室,中国疾病预防控制中心传染病预防控制所结核病控制室,北京102206

出　　处：《中国防痨杂志》2025年第3期369-373,共5页Chinese Journal of Antituberculosis

基　　金：北京市自然科学基金面上项目(7242189);中国疾病预防控制中心传染病预防控制所基金项目(08064;0104327;33066;33077);“十三五”国家科技重大专项(2018ZX10103001-003-012)。

摘　　要：病原学检测方法是目前临床实验室广泛应用的结核病诊断方法,然而受到生物样本载菌量低的限制导致检测方法的敏感度及阳性检出率尚不能满足临床快速、准确的需求。应用富集技术对生物样本中的结核分枝杆菌进行富集后检测,对提高结核病病原学检测方法的敏感度和准确度具有重要意义。笔者通过对结核分枝杆菌富集技术研究文献进行综述,为新型富集技术的开发及应用提供参考,期待开发出更多高效的富集方法,提高结核病病原学检测的敏感度,以做到早发现、早诊断、早治疗,减少结核病的传播及不良预后的发生。Diagnostic methods for tuberculosis,particularly those based on pathogen detection,are extensively employed in clinical laboratories.However,these methods often fail to meet the clinical demands for rapid and accurate diagnosis,primarily due to the challenge posed by the low bacterial load in biological samples.The integration of enrichment technologies for Mycobacterium tuberculosis detection holds significant promise in enhancing both the sensitivity and accuracy of tuberculosis diagnostic assays,thereby improving clinical outcomes.In this review,we examine the current research on Mycobacterium tuberculosis enrichment technologies,aiming to provide valuable insights for the development and application of novel enrichment methods.We anticipate the emergence of more efficient techniques that will significantly enhance the sensitivity of tuberculosis diagnostic assays.This advancement is crucial for achieving early detection,diagnosis,and treatment,ultimately reducing tuberculosis transmission and mitigating adverse prognoses.

关 键 词：分枝杆菌 结核 细菌学技术 综述 

分 类 号：R52[医药卫生—内科学] R446.5[医药卫生—临床医学]