地毯草扩张蛋白基因AcEXPA1的克隆与表达分析  

Cloning and Expression Analysis of AcEXPA1 in Axonopus compressus

作　　者：刘莉婷 高佳淼 周鸿明 李季肤 王志勇 陈志坚[3,4] LIU Li-ting;GAO Jia-miao;ZHOU Hong-ming;LI Ji-fu;WANG Zhi-yong;CHEN Zhi-jian(College of Tropical Agriculture and Forestry,Hainan University,Haikou,Hainan Province 570228,China;Sanya Nanfan ResearchInstitute,Hainan University,Sanya,Hainan Province 572025,China;Institute of Tropical Crop Genetic Resources,Chinese Academy ofTropical Agriculture Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agricultureand Rural Affair,Haikou,Hainan Province 571101,China;National Key Laboratory for Tropical Crop Breeding,Sanya,Hainan Province572024,China)

机构地区：[1]海南大学热带农林学院,海南海口570228 [2]海南大学三亚南繁研究院,海南三亚572025 [3]中国热带农业科学院热带作物品种资源研究所/农业农村部华南作物基因资源与种质创制重点实验室,海南海口571101 [4]热带作物生物育种全国重点实验室,海南三亚572024

出　　处：《草地学报》2025年第2期351-359,共9页Acta Agrestia Sinica

基　　金：海南省自然科学基金项目(321RC645);财政部和农业农村部:国家现代农业产业技术体系(CARS-34)资助。

摘　　要：为探索α-扩张蛋白(α-expansin,EXPA)基因参与铝毒害下对植物生长发育的调控作用,本研究分析了铝处理对地毯草(Axonopus compressus)生长的影响,克隆了地毯草AcEXPA1基因,并进行了生物信息学和基因表达分析。结果表明:与不加铝相比,铝处理抑制了地毯草根系生长,降低了总根长、根表面积和根体积。地毯草AcEXPA1基因全长为774 bp,编码257个氨基酸残基,蛋白分子量为27.7 kDa。AcEXPA1蛋白具有信号肽,被预测定位于胞外基质。实时定量PCR结果表明,AcEXPA1基因在地毯草根中的表达量高于叶中的表达量,且在距离根尖1~2 cm根段中的表达量最高。铝处理增强了AcEXPA1基因在地毯草根中的表达,但镉和镧处理对基因表达无显著影响。不同铝浓度和时间处理结果进一步表明铝增强了AcEXPA1基因的表达。本研究结果为解析地毯草适应铝毒害的机理提供了候选基因资源。To explore the roles ofα-expansin(EXPA)gene in regulation of plant growth and development under aluminum(Al)toxicity,the effects of Al treatments on the growth of carpetgrass(Axonopus compres⁃sus)were analyzed.And AcEXPA1 was cloned for bioinformatic and gene expression analyses.The results showed that Al toxicity inhibited carpetgrass root growth,as reflected by reduced total root length,root surface area and root volume,compared to the control without Al.The full length of AcEXPA1 is 774 bp,encoding 257 amino acid residues.Molecular weight of AcEXPA1 is 27.7 kDa.The AcEXPA1 protein includes a signal peptide and was predicted to be localized to the extracellular matrix.Real-time quantitative PCR analysis showed that AcEXPA1 exhibited higher expression in roots than in leaves,and AcEXPA1 mainly expressed in 1~2 cm root segment away from the root tip.The expression of AcEXPA1 was significantly enhanced in root of carpetgrass by Al but not cadmium and lanthanum treatments.Moreover,the results from different Al concentration and duration treatment confirmed that Al enhanced the expression of AcEXPA1 in carpetgrass roots.This study provides a candidate gene resource for dissecting the Al tolerance mechanisms in carpetgrass.

关 键 词：地毯草 铝毒害 基因表达 扩张蛋白 

分 类 号：S688.4[农业科学—观赏园艺]