玉米黄花叶病毒运动蛋白的特征分析及其在昆虫细胞内的亚细胞定位  

Characterization of maize yellow mosaic virus movement protein and its subcellular localization in insect cells

作　　者：郭思思 任春梅[1] 李硕[1] 季英华[1] 王海涛 程兆榜[1] GUO Sisi;REN Chunmei;LI Shuo;JI Yinghua;WANG Haitao;CHENG Zhaobang(Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Food Quality and Safety of Jiangsu Province,Nanjing 210014,China;College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]江苏省农业科学院植物保护研究所/江苏省食品质量安全重点实验室 [2]福建农林大学植物保护学院

出　　处：《江苏农业学报》2025年第1期35-40,共6页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(32001871)。

摘　　要：玉米黄花叶病毒(Maize yellow mosaic virus,MaYMV)是一种具有潜在流行风险的植物病毒,然而目前关于其蛋白质的特征及亚细胞定位情况研究较少。本研究选用2022年采自江苏省的MaYMV作为研究对象,对其运动蛋白(Movement protein,MP)进行分析,结果发现MP为相对分子量21 230的亲水性蛋白质,该蛋白质不含核定位信号、信号肽和跨膜区,二级结构主要呈无规则卷曲形式,且具有Luteo_Vpg功能域。利用Bacmids-MaYMV-MP-His重组穿梭质粒表达系统发现,MP定位于细胞质,进一步通过蛋白质印迹(Western-blot)法验证了MP的表达。该研究探明了MaYMV MP的特征及其亚细胞定位,有望为后续进一步分析MaYMV MP功能及其与介体蚜虫之间的相互作用奠定基础。Maize yellow mosaic virus(MaYMV) is a plant virus with potential epidemic risks.However,there are few studies on its protein characteristics and subcellular localization.In this study,MaYMV collected from Jiangsu province in 2022 was selected as the research object,and its movement protein(MP) was analyzed.The results showed that MP was a hydrophilic protein with a relative molecular weight of 21 230.The protein did not contain a nuclear localization signal,a signal peptide,or a transmembrane region.Moreover,the secondary structure was mainly in the form of random coils and had a Luteo_Vpg functional domain.Using the Bacmids-MaYMV-MP-His recombinant shuttle plasmid expression system,it was found that MP was localized in the cytoplasm.The expression of MP was further verified by Western-blot.This study demonstrated the characteristics and subcellular localization of MaYMV MP.The results of this study can lay a foundation for further analysis of the function of MaYMV MP and its interaction with vector aphids.

关 键 词：玉米黄花叶病毒 运动蛋白 亚细胞定位 

分 类 号：S435.13[农业科学—农业昆虫与害虫防治]