鸡Fnip1基因克隆、组织表达及生物信息学分析  

Cloning,tissue expression and bioinformatics analysis of chicken Fnip1 gene

作　　者：黄正洋[1] 孔令琳 王钱保[1] 李春苗[1] 吴兆林[1] 黄华云[1] 赵振华[1] HUANG Zhengyang;KONG Linglin;WANG Qianbao;LI Chunmiao;WU Zhaolin;HUANG Huayun;ZHAO Zhenhua(Jiangsu Institute of Poultry Science,Yangzhou 225125,China)

机构地区：[1]江苏省家禽科学研究所,江苏扬州225125

出　　处：《江苏农业学报》2025年第1期119-125,共7页Jiangsu Journal of Agricultural Sciences

基　　金：现代农业产业技术体系肉鸡体系项目(CARS-41-Z21);江苏省种业振兴揭榜挂帅项目[JBGS(2021)109];扬州市现代农业项目(YZ2023054)。

摘　　要：为了明确鸡卵泡素相互作用蛋白1基因(Fnip1)特征及其表达规律,本研究选择苏禽3号黄羽肉鸡为试验材料,运用分子克隆技术扩增了鸡Fnip1基因CDS区序列全长,对其序列特性进行了生物信息学分析,构建了系统进化树;利用RT-qPCR方法检测了Fnip1基因在鸡不同组织中的表达。结果获得鸡Fnip1基因CDS区,序列开放阅读框为3474 bp,位于第13号染色体16191415 bp至16251731 bp之间,编码1157个氨基酸。生物信息学分析结果显示,Fnip1基因有20个外显子,FNIP1蛋白含有FNIP_N、FNIP_M和FNIP_C等3个结构域;进化树显示,鸡先与鸟类聚为一类,再与哺乳动物聚为一支,在禽类上序列保守。FNIP1蛋白为亲水性蛋白,相对分子量为128310,理论等电点为5.25。蛋白质二级结构由α-螺旋(34.40%)、β-折叠(4.06%)、延伸链(13.74%)、无规则卷曲(47.80%)组成。表达分析结果显示,Fnip1基因在鸡的胸肌和腿肌中表达量显著高于其他组织。综上所述,本研究获得了鸡Fnip1基因CDS区序列全长,发现其在鸡肌肉组织中有较高表达。本研究结果可为进一步研究Fnip1基因在鸡肌肉生长发育中的分子机制研究奠定数据支撑。To clarify the characteristics and expression patterns of chicken follistatin-interacting protein 1 gene(Fnip1),Suqin No.3 yellow-feathered broilers were selected as experimental materials in this study,and the full-length sequence of chicken Fnip1 gene CDS region was amplified by molecular cloning technology.Moreover,the bioinformatics analysis of its sequence characteristics was carried out,and the phylogenetic tree was constructed.The expression of Fnip1 gene in different tissues of chickens was detected by RT-qPCR.The results showed that the CDS region of chicken Fnip1 gene was obtained.The open reading frame of the sequence was 3474 bp,which was located between 16191415 bp and 16251731 bp on chromosome 13,encoding 1157 amino acids.Bioinformatics analysis indicated that Fnip1 gene had 20 exons,and FNIP1 protein contained three domains:FNIP_N,FNIP_M and FNIP_C.The phylogenetic tree showed that chickens were first clustered with birds,and then clustered with mammals,and the sequence was conserved in birds.FNIP1 protein was a hydrophilic protein with a size of 128310 and a theoretical isoelectric point of 5.25.The secondary structure of the protein was composed ofα-helix(34.40%),β-sheet(4.06%),extended chains(13.74%)and random coils(47.80%).The expression analysis showed that the expression of Fnip1 gene in chicken breast muscle and leg muscle was significantly higher than that in other tissues.In summary,this study obtained the full-length sequence of the chicken Fnip1 gene CDS region and found that it was highly expressed in chicken muscle tissue.The results of this study can provide data support for further studies on the molecular mechanism of Fnip1 gene in chicken muscle growth and development.

关 键 词：鸡 Fnip1 基因克隆 表达分析 生物信息学分析 

分 类 号：S831.2[农业科学—畜牧学]