杆状病毒-昆虫表达系统(IBEVS)表达的动物病毒样颗粒及疫苗研究进展  

Research progress on animal virus-like particles expressed by insect cell-baculovirus expression vector system (IBEVS) and vaccines

作　　者：王炎 高婉宁 范益阳 玉妹 张德荣 柏家林 WANG Yan;GAO Wanning;FAN Yiyang;YU Mei;ZHANG Derong;BAI Jialin(Engineering Research Center for Key Technology and Industrialization of Cell-based Vaccine,Ministry of Education/Biomedical Research Center,Northwest Minzu University/Gansu Tech Innovation Center for Animal Cell,Lanzhou 730030,China;School of Life Sciences and Engineering,Northwest Minzu University,Lanzhou 730030,China)

机构地区：[1]细胞基质疫苗关键技术与产业化教育部工程研究中心/西北民族大学生物医学研究中心/甘肃省动物细胞技术创新中心,甘肃兰州730030 [2]西北民族大学生命科学与工程学院,甘肃兰州730030

出　　处：《江苏农业学报》2025年第1期203-208,共6页Jiangsu Journal of Agricultural Sciences

基　　金：兰州市人才创业创新项目(2020-RC-85);国家自然科学基金项目(31860696);西北民族大学中央高校基本科研业务费项目(31920190003)。

摘　　要：杆状病毒-昆虫表达系统(IBEVS)是一种高效的外源蛋白表达平台,具有可以容纳较大的外源基因片段、蛋白质表达量较高、支持蛋白质翻译后修饰、操作简便且安全性较高等优势,已被广泛用于兽用疫苗生产。病毒样颗粒(VLP)是一种由病毒蛋白自组装形成的纳米颗粒,由于缺乏病毒遗传物质,其安全性较高。VLP表面抗原排列高度有序,可有效诱导免疫反应,是一种新型的亚单位疫苗。本文综述了IBEVS在动物VLP疫苗中的应用现状,分析了当前IBEVS存在的问题及其解决策略,为动物VLP疫苗的进一步开发提供参考。The insect cell-baculovirus expression vector system(IBEVS) is a highly efficient expression platform for exogenous proteins.It has several advantages,including the capability to accommodate relatively large exogenous gene fragments,high levels of protein expression,support for post-translational modifications of proteins,ease of operation,and high safety.Due to these advantages,it has been widely used in the production of veterinary vaccines.Virus-like particles(VLPs) are nanoscale particles that are self-assembled from viral proteins.Since they lack viral genetic material,they exhibit a high degree of safety.The surface antigens of VLPs are arranged in a highly ordered manner,which can effectively induce an immune response.VLPs are a novel type of subunit vaccine.This article provides a comprehensive review of the current application status of IBEVS in animal VLP vaccines,analyzes the existing problems of IBEVS and the corresponding strategies for solving them,and offers a reference for the further development of animal VLP vaccines.

关 键 词：病毒样颗粒 杆状病毒-昆虫表达系统(IBEVS) 动物病毒样颗粒疫苗 

分 类 号：Q939.47[生物学—微生物学]