滇山茶‘狮子头’及‘大玛瑙’黄酮类物质的变化规律  

Change rule of flavonoids in Camellia reticulata‘Shizitou’and‘Damanao’

作　　者：雍清青 姚响 周麟 屈燕[1] YONG Qingqing;YAO Xiang;ZHOU Lin;QU Yan(Southwest Research Center for Engineering Technology of Landscape Architecture(State Forestry and Grassland Administration),Yunnan Engineering Research Center for Functional Flower Resource and Industrialization,School of Landscape Architecture and Horticulture Science,Southwest Forestry University,Kunming,Yunnan 650224,China;Kunming Greening Engineering Quality Detecting Test Station,Kunming,Yunnan 650224,China)

机构地区：[1]西南林业大学园林园艺学院,国家林业和草原局西南风景园林工程技术研究中心,云南省功能性花卉资源及产业化技术工程研究中心,云南昆明650224 [2]昆明市绿化工程质量检测站,云南昆明650224

出　　处：《西北农林科技大学学报(自然科学版)》2025年第2期59-71,共13页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家“十三五”重点研发计划项目(2019YFD1001005);云南省万人计划青年拔尖人才项目(YNWR-QNBJ-2019-211)。

摘　　要：【目的】探讨滇山茶‘狮子头’(Camellia reticulata‘Shizitou’)及其芽变品种‘大玛瑙’(C.reticulata‘Damanao’)花瓣中黄酮类化合物在花发育过程中的差异和相关基因的表达变化规律,为滇山茶的开发应用提供参考。【方法】以滇山茶‘狮子头’及其芽变品种‘大玛瑙’花蕾期和盛花期花瓣为材料,采用UPLC-MS/MS代谢组学分析技术及高通量转录组测序技术进行代谢组与转录组联合分析,筛选黄酮类差异代谢物及关键差异基因,并选取其中6条差异表达基因进行验证。【结果】在2个品种中共鉴定到78种黄酮类代谢物,且以黄酮醇、黄酮、异黄酮为主;其中有11个差异代谢物,包括表没食儿茶素、根皮苷、槲皮素等。转录组学分析发现,类黄酮生物合成、苯丙烷生物合成、植物激素信号转导通路与芽变的发生密切相关,在3条通路中共富集到53个差异表达基因。代谢组与转录组联合分析发现,AUX、HCT、CHS、ANR、MYB等基因可能参与滇山茶黄酮类化合物的合成。【结论】初步解析了2个滇山茶品种黄酮类代谢物成分差异的转录调控机制,筛选了差异表达基因。【Objective】Exploring the differences of flavonoids in the petals of Camellia reticulata‘Shizitou’and its bud mutant variety‘Damanao’(C.reticulata‘Damanao’)during flowers’development and the expression variation of related genes can provide a reference for the development and application of C.reticulata.【Method】We adopted metabolomics and transcriptomics to analyze the accumulation of flavonoids and the expression of differential genes in bicolor petals at the bud stage and full bloom stage,of the two sorts to elucidate the molecular mechanism underlying flavonoid accumulation in these bicolor flowers of C.reticulata.Six differentially expressed genes were selected for real-time fluorescence quantitative verification.【Result】The results showed that 78 flavonoid metabolites were identified in the two sorts,with flavonol,flavone and isoflavone being the main types.Eleven different metabolites were screened,including epigallocatechin,phloridzin and quercetin,et al.Transcriptome analysis showed that flavonoid biosynthesis,phenylpropane biosynthesis and plant hormone signal transduction pathway were closely related to the occurrence of bud mutation,and a total of 53 differentially expressed genes were enriched in these three pathways.The combined analysis of metabolomics and transcriptome showed that genes such as AUX,HCT,CHS,ANR,MYB may be involved in regulating the synthesis of flavonoids in C.reticulata.【Conclusion】In this study,the transcriptional regulation mechanism of the difference of flavonoid metabolites between two Camellia varieties was preliminarily analyzed,and differentially expressed genes were screened.

关 键 词：滇山茶 花发育过程 转录组 代谢组 黄酮 

分 类 号：S685.14[农业科学—观赏园艺]