不同诱导子对雷公藤发状根生长及主要次生代谢产物的影响  

Effects of different inducers on the growth and main secondary metabolites of Tripterygium wilfordii hairy roots

作　　者：王晓丽 杨莉莉 何泽东 陈蒙蒙 马志卿[1,2] 张斌 祝传书 WANG Xiaoli;YANG Lili;HE Zedong;CHEN Mengmeng;MA Zhiqing;ZHANG Bin;ZHU Chuanshu(Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education,College of Plant Protection,Northwest A&F University,Yangling,Shaanxi 712100,China;Engineering and Technology Centers of Biopesticide in Shaanxi,Yangling,Shaanxi 712100,China)

机构地区：[1]西北农林科技大学植物保护学院植物资源与病虫害治理教育部重点实验室,陕西杨凌712100 [2]陕西省生物农药工程技术研究中心,陕西杨凌712100

出　　处：《西北农林科技大学学报(自然科学版)》2025年第2期109-117,共9页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家重点研发计划项目(2020YFA0907900);国家自然科学基金项目(32102260);陕西省自然科学基金重点项目(2023-JC-ZD-11);西北农林科技大学科研启动费项目(2452023050)。

摘　　要：【目的】探究不同诱导子对雷公藤发状根生长及主要次生代谢产物(吉碱和次碱)含量的影响,为雷公藤次生代谢产物的开发利用及其生物合成途径解析提供理论依据。【方法】分别配制1/4 MS,1/2 MS,3/4 MS和MS 4种培养基,恒温避光培养雷公藤发状根35 d,测定雷公藤发状根干质量及雷公藤吉碱和次碱含量,筛选雷公藤发状根培养的最适培养基;以最适培养基培养雷公藤发状根,每7 d取样1次,共取样7次,测定发状根干质量,以培养时间为横坐标,发状根干质量为纵坐标绘制发状根生长曲线并确定添加诱导子的时间;提取和分离不同处理发状根及培养基中的雷公藤吉碱和次碱,并进行HPLC分析,探究不同诱导子对雷公藤发状根生长及吉碱、次碱含量变化的影响。【结果】培养35 d时,1/2 MS和MS培养基中雷公藤发状根干质量分别为0.86和0.81 g/瓶,且MS培养基中发状根的根系长势更好;MS培养基中发状根吉碱和次碱的含量均达到最高,分别为1070.57和331.72μg/g,因此确定MS培养基为雷公藤发状根培养的最适培养基。雷公藤发状根的干质量以及吉碱和次碱含量在培养21~28 d内迅速增长,28~35 d处于稳定阶段,35 d后生长速率出现下降的趋势,因此确定雷公藤发状根培养28 d添加诱导子。诱导试验结果表明,低浓度水杨酸(SA)促进雷公藤发状根的生长,100μmol/L SA处理吉碱和次碱的总产量分别达到(1777.09±70.02)和(592.16±85.61)μg/瓶;高浓度硝酸银(AgNO _(3))抑制了雷公藤发状根的生长,但显著提高了发状根中吉碱和次碱的产量,AgNO _(3)质量浓度为50 mg/L时,吉碱和次碱的总产量分别达到(3926.82±273.59)和(669.31±33.23)μg/瓶;5和10 g/L山梨醇对雷公藤发状根生长及吉碱和次碱含量均有促进作用,其中10 g/L山梨醇处理时吉碱总产量为(1567.84±135.78)μg/瓶,5 g/L山梨醇处理时次碱总产量为(388.68±30.11)μg/瓶;壳聚糖对雷公藤发状�【Objective】This research was conducted to explore the effects of inducers on the growth of the hairy roots of Tripterygium wilfordii and the content of major secondary metabolites such as wilforgine and wilforine,in order to provide theoretical basis for the development and utilization of secondary metabolites of Tripterygium wilfordii and their biosynthetic pathways.【Method】4 types of culture media were prepared,including 1/4 MS,1/2 MS,3/4 MS,and MS,and the hairy roots of Tripterygium wilfordii were cultivated under constant temperature and dark conditions.After 35 days of cultivation,the dry weight of the hairy roots and the content of wilforgine and wilforine in Tripterygium wilfordii were mea-sured to determine the most suitable culture medium to cultivate the hairy roots.Samples were taken every 7 days,with a total of 7 times,to measure the dry mass of the hairy roots.The growth curve of the hairy roots was drawn with the cultivation time as the x-axis and the dry mass of the hairy roots as the y-axis,to determine the time to add inducers.Wilforgine and wilforine in Tripterygium wilfordii were extracted and seperated from hairy roots and culture media in different treatment groups for HPLC analysis.The growth and the change of content of wilforgine and wilforine were measured after adding four inducers,sali-cylic acid(SA),AgNO _(3),sorbitol,and chitosan.【Result】In 1/2 MS medium and MS medium,the dry mass of hairy root dry weight reached 0.86 and 0.81 g per flask after 35 days separately respectively,and the content of wilforgine and wilforine in hairy roots reached 1070.57 and 331.72μg/g.Since the hairy roots grew strongly in MS medium,and the MS medium was chosen for further study.The characterization of growth curve showed that the biomass accumulation and the production of wilforgine and wilforine in hairy roots increased rapidly during day 21-28.The biomass and the content of wilforgine and wilforine became stable during day 28-35 and started to decrease after 35 days.Thus day 28 was chosen

关 键 词：雷公藤 发状根 诱导子 吉碱 次碱 

分 类 号：S482.3[农业科学—农药学]