PEG对球蛋白液-液相分离的影响  

The influence of PEG on the liquid-liquid phase separation of globular protein

作　　者：王晓婷 郑寓 吴梦雪 卢静鈃 杨光参[1] WANG Xiaoting;ZHENG Yu;WU Mengxue;LU Jingxing;YANG Guangcan(School of Mathematics and Physics,Wenzhou University,Wenzhou 32505,China)

机构地区：[1]温州大学数理学院,浙江温州325035

出　　处：《齐鲁工业大学学报》2025年第1期54-61,共8页Journal of Qilu University of Technology

基　　金：国家自然科学基金(12074289、11574232);浙江省自然科学基金面上项目(Y23A040004);浙江省高等教育“十四五”教学改革项目(jg20220509);浙江省研究生教育学会科研项目(2023-016);浙江省新苗(2023R451012);国家级创新创业计划(JWXC2022070)。

摘　　要：在液-液相分离(LLPS)过程中,盐离子浓度和拥挤剂对蛋白质的相互作用、折叠及聚集具有重要的影响。本研究借助光学倒置显微镜、动态光散射和Q5000超微量紫外分光光度计,深入探究了氯化钇(YCl_(3))对牛血清白蛋白(BSA)相行为的诱导作用,以及聚乙二醇(PEG)对BSA-YCl_(3)体系相分离的调控作用。研究结果显示,YCl_(3)能有效诱导BSA发生相分离,在YCl_(3)作用下,BSA形成了一个介于低临界盐浓度(C^(*))和高临界盐浓度(C^(**))之间的LLPS体系,而PEG的添加则使该体系的LLPS现象更加明显。此外,动态光散射实验数据揭示了YCl_(3)与BSA主要通过静电相互作用结合,而紫外分光光度计的数据则表明PEG不改变原体系LLPS的范围,但对体系LLPS有促进作用。During the liquid-liquid phase separation(LLPS)process,salt ion concentration and crowding agents have significant impacts on protein interaction,folding,and aggregation.This study employed an optical inverted microscope,dynamic light scattering,and a Q5000 UV-Vis spectrophotometer to delve into the inductive effect of yttrium(Ⅲ)chloride(YCl_(3))on the phase behavior of bovine serum albumin(BSA),as well as the regulatory role of polyethylene glycol(PEG)on the phase separation of the BSA-YCl_(3) system.The research findings indicate that YCl_(3) effectively induces phase separation in BSA,forming an LLPS system between the lower critical salt concentration(C^(*))and the higher critical salt concentration(C^(**)).The addition of PEG further enhances the LLPS phenomenon in this system.Additionally,dynamic light scattering experimental data reveal that YCl_(3) primarily interacts with BSA through electrostatic interactions,while spectrophotometer data suggest that PEG does not alter the LLPS range of the original system but rather promotes LLPS.

关 键 词：液-液相分离 拥挤环境 氯化钇 牛血清白蛋白 

分 类 号：TQ028[化学工程]