微透析样品中芍药苷LC-MS/MS分析除盐方法研究  

Study on desalination of paeoniflorin in microdialysis samples by LC-MS/MS

作　　者：严爱娟 杨颖欣 黄琴伟 周玉波 胡英 Yan Aijuan;Yang Yingxin;Huang Qinwei;Zhou Yubo;Hu Ying(Zhejiang Pharmaceutical Vocational University,Ningbo 315100,China;Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]浙江药科职业大学,宁波315100 [2]上海中医药大学,上海201203

出　　处：《分析仪器》2025年第1期48-52,共5页Analytical Instrumentation

基　　金：浙江省教育厅一般科研项目(Y202147817)。

摘　　要：目的:建立微透析技术结合液相色谱串联质谱(LC-MS/MS)技术分析时透析液中无机盐的去除方法。方法:以芍药苷为目标成分,采用LC-MS/MS技术以栀子苷为内标物对透析液除盐前后方法专属性、基质效应、线性范围、稳定性、精密度与准确度进行考察。结果:透析液除盐后基质效应显著降低,样品稳定性、方法日间精密度与回收率显著提高。结论:经方法学验证,本研究所建立的微透析样品除盐方法简便、准确可靠,可用于LC-MS/MS进样分析。Using paeoniflorin as the target component and geniposide as the internal standard,to investigate the specificity,matrix effect,linear range,stability,precision and accuracy of the method before and after desalination of dialysate by LC-MS/MS was investigated.After desalting the dialysate,the matrix effect was significantly reduced,and the sample stability,day precision and recovery of the method were significantly improved.The method is accurate,rapid,sensitive and can be used for LC-MS/MS analysis.

关 键 词：芍药苷 微透析 液相色谱串联质谱 除盐 

分 类 号：R284.1[医药卫生—中药学] O657.63[医药卫生—中医学]