基于中红外光谱和二维相关红外对不同产地山茱萸快速鉴别  

Rapid Identification of Corni Frucutus From Different Habitats Based on Mid-Infrared Spectroscopy

作　　者：王磊[1,2] 陈园杰 栗磊 刘永红 徐可可 余焕英 杨林林 董诚明 乔璐[1,2] WANG Lei;CHEN Yuan-jie;LI Lei;LIU Yong-hong;XU Ke-ke;YU Huan-ying;YANG Lin-lin;DONG Cheng-ming;QIAO Lu(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials,Zhengzhou 450046,China;School of Pharmacy,Henan University,Kaifeng 475000,China;Kaifeng Central Hospital,Kaifeng 475000,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]河南省道地药材生态种植工程技术研究中心,河南郑州450046 [3]河南大学药学院,河南开封475000 [4]开封中心医院,河南开封475000

出　　处：《光谱学与光谱分析》2025年第3期761-767,共7页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(82104329);河南省科技攻关基金项目(222102310182);河南省重点研发专项(231111312700);河南省科技攻关基金项目(242102310549);中国博士后科学基金特别资助项目(2014T170252)资助。

摘　　要：利用中红外光谱结合二维相关红外方法,对不同产地山茱萸的快速鉴别。运用中红外技术对三个省七个产地共30批山茱萸药材进行扫描,对原始光谱进行基线校正、平滑,二阶导数等预处理光谱进行差异性分析。同时将山茱萸药材红外光谱与马钱苷、莫诺苷、熊果酸三种标准品的红外光谱进行对比分析,建立二维红外相关图谱。利用分析软件对红外光谱数据进行主成分分析、聚类分析,并结合不同产地的气候因素对其差异成因进行分析。结果表明:七个产地平均光谱的峰位和峰形相似度较高,而二阶导数光谱和二维红外相关谱差异在2370~2400、2200~2300、1500~1650 cm^(-1)三个波段基团频率区。主成分分析结果显示不同产地的山茱萸在空间分布上相互独立。聚类分析结果显示可将七个产地划分为三个表征群,其中陕西丹凤、陕西佛坪、陕西商洛、陕西周至聚为一类,浙江临安聚为一类,河南西峡和河南栾川聚为一类。中红外光谱结合化学计量法及二维相关红外光谱可以实现对不同产地山茱萸药材进行产地快速鉴别。Mid-infrared spectroscopy combined with a two-dimensional correlation infrared method will be used to identify Corni fructose from different regions quickly.Mid-infrared technology scanned 30 batches of Corni fructose medicinal materials from seven production areas in three provinces.The original spectra were preprocessed with baseline correction and smoothing,and then the differences in average infrared and second-order derivative spectra were analyzed.At the same time,the infrared spectra of Corni fructose were compared and analyzed with the infrared spectra of three standard substances,namely login,mononucleosis,and ursolic acid,to establish a two-dimensional infrared correlation spectrum.Use analysis software to perform principal component and cluster analysis on infrared spectral data and analyze the causes of differences based on climate factors in different regions.The peak positions and shapes of the average spectra from the seven production areas have high similarity,while the differences in the second derivative spectra and two-dimensional infrared correlation spectrum results showed in three bands:2370~2400,2200~2300,and 1500~1650 cm^(-1).The results of the principal component analysis show that the spatial distribution of Corni fructose from different regions is independent of each other.The clustering analysis results show that the seven production areas can be divided into three characteristic groups,with Shaanxi Danfeng,Shaanxi Foping,Shaanxi Shangluo,and Shaanxi Zhouzhi clustered into one group,Zhejiang Lin an clustered into one group,and Henan Xixia and Henan Luanchuan clustered into one group.The combination of mid-infrared spectroscopy with chemical stoichiometry and two-dimensional correlated infrared spectroscopy can rapidly identify Corni fructose s origin from different origins.

关 键 词：中红外光谱 山茱萸 二维相关红外光谱 产地鉴别 

分 类 号：S153.6[农业科学—土壤学]