闽双色6号籽粒发育中叶酸合成代谢基因挖掘与分析  

Discovery and Analysis of Folate Anabolism Genes during Grain Development in Minshuangse No.6

作　　者：詹鹏麟 张扬[1] 林建新[1] 许静[1] 庄炜 卢和顶[1] 陈山虎[1] 廖长见[1] ZHAN Penglin;ZHANG Yang;LIN Jianxin;XU Jin;ZHUANG Wei;LU Heding;CHEN Shanhu;LIAO Changjian(Maize Research Center,Institute of Crop Research,Fujian Academy of Agricutural Sciences,Fuzhou 350013,China)

机构地区：[1]福建省农业科学院作物研究所,玉米研究中心,福州350013

出　　处：《中国细胞生物学学报》2025年第2期165-176,共12页Chinese Journal of Cell Biology

基　　金：福建省科技计划项目-省属公益类科研院所基本科研专项(批准号:2022R1031001、2023R1031001);福建省种业创新与产业化工程项目(批准号:zycxny2021006);福建省科技厅-星火项目(批准号:2023S0009)资助的课题。

摘　　要：叶酸是人体必需的维生素之一,甜玉米作为一种兼具果、蔬、粮3种特性的作物,其籽粒中叶酸含量变化及基因调控研究尚不清晰。以超甜玉米闽双色6号籽粒作为研究材料,测定籽粒发育不同阶段的叶酸含量及基因表达,进行Mfuzz基因时间聚类分析、基因集GO和KEGG富集分析,挖掘潜在的叶酸合成基因及分子调控通路。结果显示,甜玉米中叶酸含量随籽粒发育期的增长而逐渐下降,授粉后10天至20天叶酸含量在50μg/100 g以上,20天后呈现迅速下降的特点。基因时间序列表达模式分析获得6个类型基因集,其中Cluster 3基因集表达趋势与叶酸含量变化相似;GO和KEGG富集分析也发现叶酸生物合成和代谢通路被Cluster 3基因集富集。19个参与甜玉米籽粒叶酸生物合成的基因被挖掘,其中7个基因涉及以鸟苷三磷酸(guanosine triphosphate,GTP)为底物进行新化合物加工的生化反应通路;此外,发现Zm00001d031995(DHNA)、Zm00001d018733、Zm00001d023817(DHFS)和Zm00001d026549表达模式与叶酸含量变化趋势一致,Zm00001d039264和Zm00001d016866表达与叶酸含量变化趋势相反,这表明了这6个基因可能在叶酸分子调控中起着关键作用,该研究结果为未来高叶酸玉米育种提供潜在的基因资源。Folic acid is an essential vitamin for human body.The sweet maize is one of most important cereal crop,which has fruit,vegetable and food functional characteristic.However,the folate content and regulation genes are still unclear in sweet maize.The different kernel development stages of Minshuangse No.6 were used as materials to determine folate content and genes expression.The different expression genes were performed by Mfuzz time Cluster analysis.The Cluster genes were studied by GO and KEGG enrichment analyses,aiming toexplore potential folate synthesis genes and molecular regulatory pathways.The results showed that folate content in sweet maize decreased gradually with the increase kernel development time.The folic acid content were above 50μg/100 g from 10 to 20 days after pollination,and showed a rapid decline after 20 days.This study obtained six Clusters by Mfuzz time Cluster analysis,among which Cluster 3 gene expression pattern had a similar trend with folate content change.The GO and KEGG enrichment analyses also revealed that the folate biosynthesis and metabolism pathways were enriched by the Cluster 3.This study found 19 genes had involved in folate biosynthesis in sweet maize,among which seven genes were involved in GTP(guanosine triphosphate)as a substrate for processing new compounds.In addition,the expression pattern of Zm00001d031995(DHNA),Zm00001d018733,Zm00001d023817(DHFS)and Zm00001d026549 was consistent with the change trend of folate content.Meanwhile,the expression of Zm00001d039264 and Zm00001d016866 had opposited trend with folate content.Therefore,these six genes maybe play a key role in the regulation of folate molecular pathways,which provides genetic resources for high-folate breeding in sweet maize.

关 键 词：甜玉米 叶酸 籽粒发育 Mfuzz聚类 

分 类 号：S513[农业科学—作物学]