人血液蛋白质组学研究方法进展  

Progress in Research Methods of Human Blood Proteomics

作　　者：杨玉萍[1] 韩生成[1] 黄睿 YANG Yuping;HAN Shengcheng;HUANG Rui(Bejing Key Laboratory of Gene Resources and Molecular Development,Beijing Normal University,Beijing 100875,China;Key Lab of Stomatology of State Ethnic Affairs Commission,Northwest Minzu University,Lanzhou 730030,China;Key Laboratory of Environmental Ecology and Population Health in Northwest Minority Areas,Northwest Minzu University,Lanzhou 730030,China)

机构地区：[1]北京师范大学抗性基因资源与分子发育北京市重点实验室,北京100875 [2]西北民族大学口腔医学国家民委重点实验室,兰州730030 [3]西北民族地区环境生态与人群健康国家民委重点实验室,兰州730030

出　　处：《中国细胞生物学学报》2025年第2期266-275,共10页Chinese Journal of Cell Biology

基　　金：甘肃省教育厅教育科技创新项目(批准号:2022B-072);甘肃省自然科学基金(批准号:22JR5Ra189);中央高校专项资金项目(批准号:31920220112)资助的课题。

摘　　要：血液蛋白质组学在后基因组时代的生物医学研究中起着至关重要的作用,作为研究生物标志物的重要领域备受关注。研究人员通过高通量的蛋白质组学检测技术,可以分析血浆/血清中的各种蛋白质成分,包括总蛋白质组及蛋白质的翻译后修饰,为新生物标志物的发现和临床诊断方法的开发提供帮助。该文综述了血液蛋白质组学研究的背景和进展,重点介绍了基于质谱的LC-MS/MS技术及基于亲和性的Olink、SomaScan技术的两大类蛋白质鉴定技术方法,讨论了血液蛋白质组学领域的最新研究应用进展,并对其未来发展趋势进行了展望。Blood proteomics plays a crucial role in biomedical research in the post-genomic era and has attracted much attention as an important field for studying biomarkers.Through high-throughput proteomics detection techniques,various protein substances in plasma/serum can be analyzed,including the total proteome and posttranslational modifications of proteins,providing assistance for the discovery of new biomarkers and the development of clinical diagnostic methods.This article reviews the background and progress of blood proteomics research,focuses on introducing two major types of protein identification and analysis methods based on mass spectrometry,namely LC-MS/MS technology and affinity-based Olink and SomaScan technologies,discusses the latest research and application progress in the field of blood proteomics,and looks forward to its future development trends.

关 键 词：血液蛋白质组学 LC-MS/MS质谱检测 Olink技术 Somascan技术 

分 类 号：R341[医药卫生—基础医学]