基于血液代谢组筛选奶牛血氧饱和度相关代谢物及通路  

Analyses of Metabolites and Pathways Related to Hypoxic Stress in Dairy Cows Based on Blood Metabolome

作　　者：龙怡舟 娄文琦 黄上真 师睿[1] 陈功[2] 李斌[3] 次桑卓玛 徐青[2] 王雅春[1] LONG Yizhou;LOU Wenqi;HUANG Shangzhen;SHI Rui;CHEN Gong;LI Bin;CISANG Zhuoma;XU Qing;WANG Yachun(State Key Laboratory of Animal Biotech Breeding,National Engineering Laboratory for Animal Breeding,Key Laboratory of Animal Genetics,Breeding and Reproduction of Ministry of Agriculture and Rural Affairs,College of Animal Science and Technology,China Agricultural University,Beijing 100193,China;College of Life Sciences and Bioengineering,Beijing Jiaotong University,Beijing 100044,China;Institute of Animal Husbandry and Veterinary,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa 850000,China)

机构地区：[1]中国农业大学动物科技学院,农业农村部动物遗传育种与繁殖(家畜)重点实验室,畜禽育种国家工程实验室,畜禽生物育种全国重点实验室,北京100193 [2]北京交通大学生命科学与生物工程研究院,北京100044 [3]西藏自治区农牧科学院畜牧兽医研究所,拉萨850000

出　　处：《畜牧兽医学报》2025年第2期621-632,共12页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：西藏自治区科技计划项目(XZ202201ZY0004N);西藏自治区区域协同创新专项(QYXTZX-LS2021-01);西藏自治区自然科学基金项目(XZ202201ZR0010G);国家现代农业产业技术体系(CARS-36);长江学者和创新团队发展计划(IRT_15R62)。

摘　　要：旨在基于奶牛血氧饱和度(blood oxygen saturation,BOS)高、低组的血液代谢物,尝试筛选BOS相关差异代谢物及通路。本研究所用血样及个体信息采集自西藏地区两个规模化牧场的60头荷斯坦牛。使用质子核磁共振光谱(proton nuclear magnetic resonance spectroscopy,1H NMR)绝对定量法测定BOS高、低组各30头荷斯坦牛的43种血液代谢物图谱,经差异倍数分析、t检验和变量投影重要性3种方法筛选BOS相关差异代谢物并进行富集分析。结果,共筛选到17种差异代谢物,其中与低BOS组相比,高BOS组血液中甘氨酸、甘露醇和尿素浓度更高(P<0.05),柠檬酸盐、甲酸盐、丙酮酸盐等14种指标浓度更低(P<0.05)。这些差异代谢物富集到乙醛酸和二羧酸代谢,甘氨酸、丝氨酸和苏氨酸代谢等16条显著的代谢通路。差异代谢物与奶牛机体氨基酸和有机酸等多种代谢有关,起到保护组织、治疗损伤、调节细胞稳态、缓解低氧应激等积极作用。综上,本研究筛选到BOS水平相关的差异代谢物以及通路,为后续深入研究奶牛高原适应机制和缓解高原低氧应激提供了参考。This study aimed to identify differential metabolites and pathways associated with blood oxygen saturation(BOS)by analyzing blood metabolites between the high and low BOS groups of dairy cows.Blood samples were collected from 60 Chinese Holstein cows in two Tibet’s commercial farms,then 43 blood metabolite profiles and other phenotypes were obtained from 30 Holstein cows in each of the high and low BOS groups based on absolute quantification by 1H NMR.Fold Change analysis,t-test and variable importance in projection were used to investigate the differential metabolites and related pathways related to BOS.A total of 17 metabolites were found to be associated with high and low BOS,with 3 up-regulated metabolites(glycine,mannitol,urea;P<0.05)and 14 down-regulated metabolites(citrate,formate,pyruvate and others;P<0.05)in high BOS group.The 16 metabolic pathways were found that related to the glyoxylate and dicarboxylate metabolism,glycine,serine and threonine metabolism and others.Differential metabolites were related to a variety of metabolisms such as amino acids and organic acids in the cow body and had a critical role in the protection and treatment of tissue damage,the regulation of cellular homeostasis,and the alleviation of stress caused by hypoxia.In general,this study identified the differential metabolites as well as pathways related to BOS levels,providing a foundation for further research on the mechanism of high-altitude adaptation in cows and alleviating high-altitude hypoxia stress.

关 键 词：荷斯坦牛 血氧饱和度 血液代谢物 高原低氧应激 

分 类 号：S823.91[农业科学—畜牧学]