高效液相色谱同时分析荣筋壮骨方中多种化学成分  

Simultaneous Analysis of Multiple Chemical Components in Rongjin Zhuanggu Prescription by High Performance Liquid Chromatography

作　　者：秦艺菲 许惠凤 施颖 黄思贤 李家乐 王晓宁[3] 刘玲珑 李西海 王丽丽 QIN Yifei;XU Huifeng;SHI Ying;HUANG Sixian;LI Jiale;WANG Xiaoning;LIU Linglong;LI Xihai;WANG Lili(Academy of Integrated Medicine,Fujian University of Traditional Chinese Medicine,Fujian Province,Fuzhou 350122,China;Fujian Key Laboratory of Integrative Medicine on Geriatrics,Fujian University of Traditional Chinese Medicine,Fujian Province,Fuzhou 350122,China;College of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fujian Province,Fuzhou 350122,China;College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fujian Province,Fuzhou,350122,China)

机构地区：[1]福建中医药大学中西医结合研究院,福建福州350122 [2]福建省中西医结合老年性疾病重点实验室,福建福州350122 [3]福建中医药大学中西医结合学院,福建福州350122 [4]福建中医药大学药学院,福建福州350122

出　　处：《光明中医》2025年第2期291-295,共5页GUANGMING JOURNAL OF CHINESE MEDICINE

基　　金：国家自然科学基金(No.82374495,82305267);福建省自然科学基金项目(No.2022J01371);福建省卫健委科技计划项目(No.2023ZQNZD016);福建中医药大学陈可冀中西医结合发展基金项目(No.CKJ2021012);福建中医药大学校管课题(No.X2021021)。

摘　　要：目的建立荣筋壮骨方中成分提取以及高效液相色谱(HPLC)测定其多种成分的分析方法。方法采用Agilent EC-C18(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈-水,进行梯度洗脱,柱温30℃,流速为1 ml/min,检测波长为212 nm,考察甲醇、乙醇、纯水和正丁醇对荣筋壮骨方水提物冻干粉的提取效果,评估该方法的稳定性,检测该复方中几种物质的含量。结果在最优的色谱条件下,荣筋壮骨方中多种化学成分达到了较好的分离效果,且适用于荣筋壮骨方中梓醇、芍药苷、川续断皂苷Ⅵ、补骨脂素、异补骨脂素5种成分的定量分析。经测定,荣筋壮骨方中梓醇的含量为38.30 mg/g,芍药苷为8.13 mg/g,川续断皂苷Ⅵ为30.18 mg/g,补骨脂素为3.89 mg/g,异补骨脂素为2.02 mg/g。结论本研究建立的HPLC方法具有回收率高、重现性好等优点,适用于荣筋壮骨方中多种成分的定性及定量分析。Objective To establish a method for the extraction and qualitative and quantitative analysis of Rongjin Zhuanggu prescription by high performance liquid chromatography(HPLC).Methods The extraction effect of methanol,ethanol,pure water and n-butanol on the lyophilised powder of the aqueous extract of Rongshi Zhuanggu prescription was investigated on an Agilent EC-C18(4.6 mm×250 mm,5μm)column with the mobile phase of acetonitrile-water in a gradient elution mode with the column temperature of 30℃,the flow rate of 1 ml/min,and the detection wavelength of 212 nm,and the stability of the method was evaluated,and the content of several substances in the compound was finally detected.The contents of several substances in the compound were finally detected.Results Under the optimal chromatographic conditions,the separation effect of various chemical components in Rongjin Zhuanggu prescription was good,and it was suitable for the quantitative analysis of five components in Rongjin Zhuanggu prescription as follows:catalpa,paeoniflorin,paracosaponinⅥ,psoralen and isopsoralen.The content of catalpol,paeoniflorin,paeoniflorinⅥ,psoralen and isopsoralen were 38.30 mg/g,8.13 mg/g,30.18 mg/g,3.89 mg/g and 2.02 mg/g respectively in Rongjin Zhuanggu prescription.Conclusion The HPLC method established in this study has the advantages of high recovery and good reproducibility,and is suitable for the qualitative and quantitative analysis of various components in Rongjin Zhuanggu prescription.

关 键 词：荣筋壮骨方 中药成分分析 高效液相色谱 

分 类 号：R28[医药卫生—中药学]