2型糖尿病浊毒内蕴证患者血浆代谢组学特征分析  

Analysis of plasma metabonomic characteristics of type 2 diabetes mellitus patients with turbid toxin accumulation syndrome

作　　者：赵子琪 庞湃 任越 王斌[1] 马运涛[1] 杨千晶 吴深涛[1] ZHAO Ziqi;PANG Pai;REN Yue;WANG Bin#;MA Yuntao;YANG Qianjing;WU Shentao(First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,Tianjin 300381,China)

机构地区：[1]天津中医药大学第一附属医院,国家中医针灸临床医学研究中心,天津300381

出　　处：《北京中医药大学学报》2025年第1期34-42,共9页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(No.82474450,No.81273710,No.81704008);全国名老中医药专家传承工作室建设项目(No.国中医药发[2022]75号);2024年天津市南开区中医药传承创新发展示范试点项目-重点专科建设-内分泌科(No.20240318)。

摘　　要：目的探讨2型糖尿病浊毒内蕴证患者血浆代谢组学特征。方法纳入2023年11月—2024年2月就诊于天津中医药大学第一附属医院的2型糖尿病浊毒内蕴证患者103例,同时招募健康受试者54名,收集2组人群一般情况,运用超高效液相色谱-静电场轨道阱高分辨质谱法检测血浆代谢物含量,构建正交偏最小二乘法判别分析模型,筛选组间变化明显的代谢产物,以代谢物第一主成分的变量重要性投影≥1、|log_(2)FC|>1、P<0.05为标准筛选差异代谢物。选用内部数据库和人类代谢组数据库对差异代谢物进行注释,MetaboAnalyst网站进行通路分析。结果2组性别、年龄差异无统计学意义。筛选出潜在差异代谢物17种,其中2型糖尿病浊毒内蕴证患者D-4′-磷酸泛酸、2,6-二氯吲哚酚、4-甲基苯酚、次黄嘌呤、11,12-环氧二十碳三烯酸、油酰胺、3-苯基乳酸高于健康人(P<0.05);3-甲氧基苯甲酸、3-碘十八烷酸、甲苯咪唑、β-丙氨酸、柠檬酸、反式乌头酸、香叶基二磷酸酯、溶血磷脂酰胆碱(18∶2)、磷脂酰乙醇胺(18∶1)、己内酰胺低于健康人(P<0.05)。共获得10条代谢通路,其中关键代谢通路为泛酸和辅酶A生物合成代谢通路。结论2型糖尿病浊毒内蕴证患者与健康人群存在代谢差异,其内在机制可能与D-4′-磷酸泛酸及β-丙氨酸所共同参与的泛酸和辅酶A生物合成代谢通路有关。Objective To explore the plasma metabonomic characteristics of patients with type 2 diabetes mellitus and turbid toxin accumulation syndrome.Methods One hundred and three patients with type 2 diabetes mellitus and turbid toxin accumulation syndrome were enrolled from November 2023 to February 2024 in the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine and 54 healthy individuals were recruited.The general data of the two groups were analyzed,and the plasma metabolite content was detected using ultra-high performance liquid chromatography-Orbitrap mass spectrometry.Construct an orthogonal partial least squares discriminant analysis model to screen metabolites with significant intergroup changes.The variable importance in projection≥1,|log_(2)FC|>1,and P<0.05 were used as the criteria for the screening of differential metabolites.Annotate differential metabolites using internal databases and the human metabolome database,and perform pathway analysis using MetaboAnalyst website.Results There was no statistically significant difference in gender and age between the two groups.Seventeen potential differential metabolites were identified.The D-4'-phosphopantothenate,2,6-dichloroindophenol,4-methylphenol,hypoxanthine,11,12-epoxyeicosatrienoic acids,oleamide,3-phenyllactic acid contents were higher in patients with type 2 diabetes mellitus and turbid toxin accumulation syndrome than in healthy individuals(P<0.05);3-anisic acid,3-iodooctadecanoic acid,mebendazole,β-alanine,citric acid,trans-aconitic acid,geranyl diphosphate,lysophosphatidylcholine(18∶2),phosphatidylethanolamine(18∶1),and caprolactam contents were lower in patients with type 2 diabetes mellitus and turbid toxin accumulation syndrome than in healthy individuals(P<0.05).Ten metabolic pathways were identified,including the key metabolic pantothenate and coenzyme A biosynthesis pathways.Conclusion Metabolic differences were observed between patients with type 2 diabetes mellitus and turbid toxin accumulation syndrome and hea

关 键 词：2型糖尿病 浊毒内蕴证 代谢组学 血浆生物标志物 代谢通路 

分 类 号：R255.4[医药卫生—中医内科学]