肥胖男童尿液差异代谢物的筛选  

Screening of differential metabolites in the urine of obese boys

作　　者：刘韦 董洁 高超男 齐千瑾 孔亚伟 孟鑫 彭晓霞[3] 闫银坤 LIU Wei;DONG Jie;GAO Chaonan;QI Qianjin;KONG Yawei;MENG Xin;PENG Xiaoxia;YAN Yinkun(Center for Non-communicable Disease Management,Beijing Children′s Hospital,Capital Medical University,National Center for Children′s Health,Beijing 100045,China;Pediatric Medical Research Institute,Hunan Children′s Hospital,Changsha,Hunan 410000,China;Center for Clinical Epidemiology and Evidence-Based Medicine,BeijingChildren′s Hospital,Capital Medical University,National Center for Children′s Health)

机构地区：[1]首都医科大学附属北京儿童医院慢病中心,北京100045 [2]湖南省儿童医院儿科医学研究所,湖南长沙410000 [3]首都医科大学附属北京儿童医院临床流行病与循证医学中心

出　　处：《中国儿童保健杂志》2025年第2期180-184,190,共6页Chinese Journal of Child Health Care

基　　金：北京新星计划(Z201100006820008);湖南省自然科学基金(2022J40198);国家自然科学基金(81803254,81973110,82073572)。

摘　　要：目的探究肥胖男童与体重正常男童之间尿液代谢物特征的差异,为肥胖的发病机制提供初步线索。方法采用病例对照研究设计,于2021年9—10月在湖南长沙三所寄宿学校招募6~14岁儿童,按体重状态分为肥胖组和体重正常组,将两组儿童按年龄1∶1进行匹配,最终纳入10名正常体重男童和10名肥胖男童。连续收集3d 24h尿样,混匀后取2mL尿样进行靶向检测。采用正交偏最小二乘法判别分析比较两组间代谢物的整体组成差异、t检验和差异倍数值相结合的方法筛选差异代谢物。结果肥胖组中吲哚-3-甲基乙酸酯、吲哚-3-丙酸、吲哚乙酸、胆酸、乙酰胆碱酸、石胆酸-3-硫酸盐、7-酮去氢胆酸、甲基半胱氨酸、脯氨酸和癸二酸的水平低于正常体重组,差异有统计学意义(Log_(2)FC≤-0.5,P<0.05)。结论肥胖男童与体重正常男童的尿液代谢物存在差异,可为肥胖的预防和控制提供新线索。Objective To investigate the differences in urinary metabolite profiles between obese and normal-weight boys,in order to provide preliminary clues for understanding the pathogenesis of obesity.Methods A case-control study design was employed.From September to October 2021,children aged 6-14 years were recruited from three boarding schools in Changsha,Hunan.Participants were divided into obese and normal-weight groups based on their weight status.Ten normal-weight boys and ten obese boys were matched by age(1∶1 ratio).24-hour urine samples were collected continuously for three days,mixed,and 2mL of urine was taken for targeted detection.Orthogonal partial least squares discriminant analysis(OPLS-DA)was used to compare the overall differences in metabolite composition between the two groups,and a combination of t-test and fold change(FC)values was employed to screen for differential metabolites.Result The levels of Indole-3-methyl acetate,Indole-3-propionic acid,Indoleacetic acid,Cholic acid,Glycocholic acid,Sulfated lithocholic acid,7-dehydrocholic acid,Methylcysteine,Proline,and Sebacic acid were significantly lower in the obese group compared to the normal-weight group(Log_(2)FC≤-0.5,P<0.05).Conclusion Differences in urine metabolites exist between obese and normal-weight male children,which may provide new clues for the prevention and control of obesity.

关 键 词：肥胖 差异代谢物 尿液 代谢组学 

分 类 号：R723.14[医药卫生—儿科]