基于靶向检测分析大黄素对正常小鼠肝组织中脂肪酸代谢的影响  

Effect of emodin on fatty acid metabolism in normal mouse liver tissues based on targeted detection

作　　者：毛睿 赵付霞 蔡亚楠 任超云 张紫媛 王颖莉 Mao Rui;Zhao Fuxia;Cai Yanan;Ren Chaoyun;Zhang Ziyuan;Wang Yingli(Experimental Management Center of Shanxi University of Chinese Medicine,Jinzhong Shanxi 030619)

机构地区：[1]山西中医药大学实验管理中心,山西晋中030619

出　　处：《山西中医药大学学报》2025年第2期144-151,共8页Journal of Shanxi University of Chinese Medicine

基　　金：山西省中医药管理局省教育规划办项目(2023ZYYB2035);山西省基础研究计划自由探索青年项目(202303021212238);山西中医药大学博士科研启动基金项目(2023BK12);山西中医药大学优秀博士毕业生来晋工作奖励经费科研启动基金项目(2023BKS21)。

摘　　要：目的:构建小鼠肝组织中脂肪酸(FFA)含量的靶向检测方法,并进行方法学考察,为大黄素的临床应用提供科学数据。方法:将32只小鼠随机分为空白组(C组)、大黄素低剂量组(C_L组)、大黄素中剂量组(C_M组)、大黄素高剂量组(C_H组),每组8只。大黄素给药剂量分别为40 mg/kg,80 mg/kg,120 mg/kg,1次/d,连续给药4周。采用超高效液相色谱-质谱(UHPLC-MS)技术检测小鼠肝组织中FFA含量的变化,利用SIMCA 14.1软件对原始数据进行主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA),比较FFA含量的组间差异,根据变量权重值(VIP)>1.0筛选差异代谢物,通过热图进行可视化分析,明确大黄素对小鼠FFA代谢的影响。结果:C_L组小鼠肝组织中FFA含量与C组比较,差异无统计学意义(P>0.05);而C_M组与C_H组小鼠肝组织中亚油酸[FFA(18∶2)]、花生四烯酸[FFA(20∶4)]含量明显高于C组,差异有统计学意义(P<0.01),即C_M组与C_H组小鼠肝组织中Omega-6多不饱和脂肪酸(n-6 PUFAs)产生堆积。与C组比较,C_L组、C_M组与C_H组小鼠肝组织中FFA(20∶4)/二十二碳六烯酸[FFA(22∶6)]、棕榈酸[FFA(16∶0)]/FFA(22∶6)升高,差异有统计学意义(P<0.01)。结论:FFA(18∶2)、FFA(18∶0)、FFA(18∶1)、FFA(16∶0)、FFA(20∶4)、FFA(20∶4)/FFA(22∶6)、FFA(16∶0)/FFA(22∶6)可能是大黄素调节肝组织FFA代谢的潜在生物标志物。Objective:To establish a targeted detection method for fatty acid(FFA)content in mouse liver tissues,investigate the methodology,and provide scientific data for the clinical application of emodin.Methods:32 mice were randomly divided into blank group(C),low-dose emodin group(C_L),medium-dose emodin group(C_M),high-dose emodin group(C_H),with 8 mice in each group.Emodin was administered at 40 mg/kg,80 mg/kg and 120 mg/kg once a day for 4 weeks.Ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS)was used to detect the changes of FFA content in mouse liver tissues.Principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were performed on the original data using SIMCA 14.1 software to compare the difference of FFA content between groups.Differential metabolites were screened according to variable importance projection(VIP)>1.0.The effects of emodin on FFA metabolism in mice were visualized by heat map analysis.Results:There was no statistical significance in FFA content in mouse liver tissues between C_L group and C group(P>0.05).The contents of Linoleic acid[FFA(18:2)]and arachidonic acid[FFA(20:4)]in mouse liver tissues in C_M group and C_H group were significantly higher than those in C group,and the difference was statistically significant(P<0.01),which indicated that Omega-6 polyunsaturated fatty acids(n-6 PUFAs)were accumulated in mouse liver tissues in C_M and C_H groups.Compared with those of C group,FFA(20:4)/docosahexaenoic acid[FFA(22:6)]and palmitic acid[FFA(16:0)]/FFA(22:6)in mouse liver tissue in C_L group,C_M group and C_H group were increased,and the differences were statistically significant(P<0.01).Con⁃clusion:FFA(18:2),FFA(18:0),FFA(18:1),FFA(16:0),FFA(20:4),FFA(20:4)/FFA(22:6),and FFA(16:0)/FFA(22:6)may be potential biomarkers of emodin regulation of FFA metabolism in liver tissues.

关 键 词：大黄素 脂肪酸 靶向检测 肝组织 

分 类 号：R285.5[医药卫生—中药学]