马钱子苷通过LncRNA MALAT1靶向microRNA-155-5p促进雪旺细胞增殖和迁移的机制研究  

Loganin promotes Schwann cell proliferation and migration by targeting microRNA-155-5p through lncRNA MALAT1

作　　者：赵飞 姚忠军 方兴刚 张弥 Zhao Fei;Yao Zhong-jun;Fang Xing-gang;Zhang Mi(Department of Orthopaedics II,Taihe Hospital,Hubei University of Medicine,Shiyan,Hubei 442000,China;Department of Integrated Traditional Chinese and Western Medicine,Taihe Hospital,Hubei University of Medicine,Shiyan,Hubei 442000,China;Department of Dermatology,People's Hospital Affiliated to Hubei University of Medicine,Shiyan,Hubei 442000,China)

机构地区：[1]十堰市太和医院(湖北医药学院附属医院)骨Ⅱ科,湖北十堰442000 [2]十堰市太和医院(湖北医药学院附属医院)中西医结合科,湖北十堰442000 [3]湖北医药学院附属人民医院皮肤科,湖北十堰442000

出　　处：《中国现代医学杂志》2025年第4期22-29,共8页China Journal of Modern Medicine

基　　金：湖北省卫健委青年人才项目(No:WJ2021F032)。

摘　　要：目的探究马钱子苷(Loganin)通过LncRNA MALAT1靶向microRNA-155-5p(miR-155-5p)促进雪旺细胞增殖和迁移的机制研究。方法以小鼠雪旺细胞为研究对象,实验设置对照组(oe-NC组、siNC组、Control组)、LncRNA MALAT1过表达组(oe-MALAT1组)、LncRNA MALAT1干扰组(si-MALAT组)、共转染oe-MALAT1+mimic NC组或oe-MALAT1+miR mimic(miR-155-5p过表达)组;用50、100和200μmol/L浓度的Loganin处理细胞,分为Loganin(50μmol/L)组、Loganin(100μmol/L)组、Loganin(200μmol/L)组;细胞转染si-NC或si-MALAT后用100μmol/L Loganin处理,分为Loganin(100μmol/L)+si-NC组和Loganin(100μmol/L)+si-MALAT组。通过实时荧光聚合酶链反应(qRT-PCR)检测雪旺细胞中LncRNA MALAT1和miR-155-5p的表达;利用CCK-8实验和Transwell实验检测oe-NC组、oe-MALAT1组、oe-MALAT1+mimic NC组及oe-MALAT1+miR mimic组雪旺细胞增殖和迁移能力。通过荧光素酶报告实验验证LncRNA MALAT1与miR-155-5p的靶向关系。qRT-PCR检测不同浓度的Loganin(50、100和200μmol/L)对雪旺细胞中LncRNAMALAT1表达的影响。通过CCK-8实验和Transwell实验检测不同浓度的Loganin组、Loganin(100μmol/L)+si-NC组和Loganin(100μmol/L)+siMALAT组中雪旺细胞增殖和迁移的能力。结果与oe-NC组比较,oe-MALAT1组中LncRNA MALAT1水平升高(P<0.05);且oe-MALAT1组雪旺细胞的增殖和迁移能力增强(P<0.05)。荧光素酶报告实验证实LncRNA MALAT1靶向负调节miR-155-5p,抑制MALAT1的水平可促进miR-155-5p的表达(P<0.05)。与oeMALAT1+mimic NC组组比较,oe-MALAT1+miR mimic组雪旺细胞增殖和迁移能力减弱(P<0.05)。与Control组比较,不同浓度的Loganin促进雪旺细胞增殖和迁移(P<0.05),且具有浓度效应。此外,Loganin显著促进LncRNA MALAT1的表达(P<0.05)。相对于Loganin(100μmol/L)+si-NC组,Loganin(100μmol/L)+siMALAT组雪旺细胞的增殖和迁移能力被抑制(P<0.05)。结论Loganin通过上调LncRNA MALAT1而抑制miR-155-5p的表达,促进雪旺细胞的增殖和迁移,为坐骨神经Objective To explore the whether loganin promotes Schwann cell proliferation and migration by targeting microRNA-155-5p through lncRNA MALAT1.Methods Using mouse Schwann cells as the research model,the experiment was designed with the following groups:control groups(oe-NC and si-NC groups),lncRNA MALAT1 overexpression group(oe-MALAT1 group),lncRNA MALAT1 interference group(si-MALAT1 group),oe-MALAT1+mimic NC co-transfection group(oe-MALAT1+mimic NC group)and oe-MALAT1+miR mimic co-transfection group(miR-155-5p overexpression group).Cells were treated with different concentrations of loganin(50,100,and 200μmol/L)and divided into loganin(50μmol/L),loganin(100μmol/L),and loganin(200μmol/L)groups,and cells transfected with si-NC or si-MALAT were treated with 100μmol/L loganin and divided into loganin(100μmol/L)+si-NC group and loganin(100μmol/L)+si-MALAT group.The expression levels of lncRNA MALAT1 and miR-155-5p in Schwann cells were detected by quantitative real-time polymerase chain reaction(qRT-PCR),and the proliferation and migration abilities of Schwann cells in the oe-NC group,the oe-MALAT1 group,the oe-MALAT1+mimic NC group,and the oe-MALAT1+miR mimic group were determined using the CCK-8 assay and the Transwell assay.The targeting relationship between lncRNA MALAT1 and miR-155-5p was verified by luciferase reporter assay.The qRT-PCR was performed to detect the effects of different concentrations of loganin(50,100,and 200μmol/L)on the expression of lncRNA MALAT1 in Schwann cells.The ability of Schwann cells to proliferate and migrate in different concentrations of loganin,and that in the loganin(100μmol/L)+si-NC group and the loganin(100μmol/L)+si-MALAT group,were detected using the CCK-8 assay and the Transwell assay.Results Compared with the oe-NC group,the level of lncRNA MALAT1 in the oe-MALAT1 group increased significantly(P<0.05).Moreover,the proliferation and migration abilities of Schwann cells in the oe-MALAT1 group were significantly enhanced(P<0.05).The luciferase reporter assay confirmed

关 键 词：马钱子苷 雪旺细胞 LncRNA MALAT1 microRNA-155-5p 增殖 迁移 

分 类 号：R446.6[医药卫生—诊断学]