何首乌饮片表面特征的数字表征及与化学成分含量的相关性研究  

Digital Characterization of Surface Characteristics of Polygonum multiflorum Thunb.Pieces and Correlations with Chemical Composition Contents

作　　者：杨颜榕 石佳 李妍怡 魏锋[2] 马双成 王芳 汪祺[2] YANG Yanrong;SHI Jia;LI Yanyi;WEI Feng;MA Shuangcheng;WANG Fang;WANG Qi(Shenyang Pharmaceutical University,School of Functional Food and Wine,Shenyang 110016,China;National Institutes for Food and Drug Control,Institute for Control of Chinese Traditional Medicine and Ethnic Medicine,Beijing 100050,China;School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;Chinese Pharmacopoeia Commission,Beijing 100061,China;State Key Laboratory of Drug Regulatorg Science,Beijing 100061,China)

机构地区：[1]沈阳药科大学功能食品与葡萄酒学院,沈阳110016 [2]中国食品药品检定研究院中药民族药检定所,北京100050 [3]北京中医药大学中药学院,北京102400 [4]国家药典委员会,北京100061 [5]药品监管科学全国重点实验室,北京100061

出　　处：《中国药物警戒》2024年第12期1363-1370,共8页Chinese Journal of Pharmacovigilance

基　　金：国家自然科学基金资助项目(82374033)。

摘　　要：目的对何首乌饮片表面特征进行数字化表征,并探究何首乌饮片表面特征色度值与其5个主要化学成分含量的相关性,建立一种基于其表面特征色度值,即饮片表面颜色初步判断何首乌药材质量的方法。方法采用体视荧光显微镜对何首乌不同部位的表面特征进行图像采集,利用Photoshop CS6(中文版)获取色度值R、G、B、L^(*)、a^(*)、b^(*);采用超高效液相色谱-三重四极杆质谱法(UPLC-QQQ-MS/MS)测定何首乌中二苯乙烯苷、大黄素、大黄素-8-O-β-D-葡萄糖苷、大黄素甲醚、大黄素甲醚-8-O-β-D-葡萄糖苷5个成分的含量,应用聚类分析(HCA)、单因素方差分析以及Pearson相关性分析方法分析何首乌色度值与5个成分含量的相关性。结果HCA结果显示不同产地的何首乌饮片聚为3类,分别是饮片断面颜色为浅黄白色、黄棕色与红棕色,表明不同产地何首乌饮片颜色有差异;单因素方差分析结果显示,断面不同颜色的何首乌饮片二苯乙烯苷和大黄素的含量具有显著差异:宏观上,何首乌饮片断面颜色浅黄白色者二苯乙烯苷含量较高,断面颜色呈黄棕色者大黄素含量较高,饮片断面呈红棕色者二苯乙烯苷和大黄素含量较低。相关性分析结果显示,何首乌饮片皮部色度值与相关含量也存在一定规律,何首乌饮片皮部色度值a与大黄素甲醚含量呈负相关关系。结论该方法具有准确性、便捷性;色度值量化为何首乌的经验鉴别提供了数据支撑,揭示饮片断面颜色与何首乌中相关化学成分的关联性是对何首乌传统鉴别经验的科学阐释,更是传统经验与现代科学的有机结合。Objects To establish a method for evaluating the quality of Polygonum multiflorum Thunb.pieces based on the surface characteristic chromaticity value,that is,the surface color of Polygonum multiflorum Thunb.Methods The surface features of different parts of Polygonum multiflorum Thunb.were collected under a stereo fluorescence microscope,and the chromaticity values of R,G,B,L^(*),a^(*) and b^(*) were obtained with photo shop cs6 software.The contents of stilbene glucoside,emodin,emodin-8-O-β-D-glucoside,emodin methyl ether and emodin methyl ether-8-O-β-D-glucoside in Polygonum multiflorum Thunb.were determined by UPLC-QQ-MS/MS,and the correlations between the chromaticity value of Polygonum multiflorum Thunb.and the contents of the five components were analyzed via cluster analysis(HCA),one-way ANOVA and Pearson correlation analysis.Results The results of HCA showed that Polygonum multiflorum Thunb.pieces from different habitats were grouped into three categories,which were light yellow-white,yellow-brown and reddish-brown,indicating that the colors of Polygonum multiflorum Thunb.pieces from different habitats varied.The results of one-way ANOVA showed that there were significant differences in the contents of stilbene glycoside and emodin in Polygonum multiflorum Thunb.pieces with different cross sections.Macroscopically,the stilbene glycoside content was higher in Polygonum multiflorum Thunb.pieces with light yellow and white cross sections,emodin content was higher in those with yellow-brown cross sections,and the content of stilbene glycoside and emodin was lower in those with red-brown cross sections.The results of correlation analysis showed that there was also a correlation between the chromaticity value and the related content of Polygonum multiflorum Thunb.The skin chromaticity value A of Polygonum multiflorum Thunb.was negatively correlated with the content of emodin methyl ether.Conclusion The established method is accurate and user-friendly.The quantification of chromaticity values can provide data

关 键 词：何首乌 超高效液相色谱-三重四级杆质谱法 二苯乙烯苷类成分 蒽醌类成分 性状 色度 化学成分 数字化 

分 类 号：R927.2[医药卫生—药学] R917