不同砧木嫁接番茄对酶活和激素含量的影响  

Effects of Different Rootstocks Grafting Tomato onEnzyme Activity and Hormone Content

作　　者：梅文宇 黎振兴[1] 陈兴平 宫超 孙保娟[1] 徐伟杰 曾果 李涛[1] MEI Wenyu;LI Zhenxing;CHEN Xingpin;GONG Chao;SUN Baojuan;XU Weijie;ZENG Guo;LI Tao(Vegetable Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for New Technology Research of Vegetables,Guangzhou 510640,China;Ganzhou Vegetable and Flower Research Institute/Jiangxi Facility Vegetable Technology Innovation Center,Ganzhou 341400,China;Guangdong Province Seed Introduction Service Company,Zhuhai 519020,China;Xingguo County Agricultural Comprehensive Administrative Enforcement Brigade,Ganzhou 342400,China)

机构地区：[1]广东省农业科学院蔬菜研究所/广东省蔬菜新技术研究重点实验室,广东广州510640 [2]赣州市蔬菜花卉研究所/江西省设施蔬菜技术创新中心,江西赣州341400 [3]广东省良种引进服务公司,广东珠海519020 [4]兴国县农业综合行政执法大队,江西赣州342400

出　　处：《广东农业科学》2024年第12期64-75,共12页Guangdong Agricultural Sciences

基　　金：国家重点领域研发计划项目(2023YFD1201504);广东省乡村振兴战略专项(2024-NPY-00-021);广东省重点领域研发计划项目(2020B020220001);广东省农业科学院科技创新战略专项(高水平农科院建设)(R2020PY-JX003)。

摘　　要：【目的】研究茄果类作物嫁接的亲和性表现,探究不同砧木嫁接番茄接穗后接口处的生理生化反应。【方法】以番茄接穗自嫁接(TS/TS)为对照,与番茄接穗嫁接番茄砧木(TS/TR)、番茄接穗嫁接茄子砧木(TS/ER)、番茄接穗嫁接辣椒砧木(TS/PR)进行对比,系统评估各嫁接组合在嫁接后不同时间节点的多酚氧化酶(PPO)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性,总酚、总类黄酮、丙二醛(MDA)、生长素(IAA)、细胞分裂素(CTK)及赤霉素(GA)、脱落酸(ABA)、茉莉酸(JA)、水杨酸(SA)含量变化。【结果】研究发现,TS/TS、TS/TR、TS/ER组合亲和性较好,TS/PR组合亲和性差,其中TS/PR组合在嫁接24 d后接口处总酚、总类黄酮、MDA含量分别为2.26 mg/g、1.88 mg/g、6.51 μmol/L,而TS/TS组合下三者分别为0.65 mg/g、0.26 mg/g、2.66 μmol/L。自嫁接及亲和性较好的TS/TR、TS/ER组合,接口处总酚、总类黄酮、MDA含量显著低于TS/PR组合,接口氧化应激程度较小。而TS/TR、TS/ER组合在生长阶段接口处总酚、总类黄酮、MDA含量显著高于TS/TS组合。对接口处酶活性及相关物质含量进行主成分分析,发现同种嫁接与异种嫁接在接口处氧化相关指标上具有显著差异。测定不同嫁接组合接口处6种激素后发现,自嫁接接口处CTK含量最低、JA含量最高,TS/PR组合接口CTK含量最高、JA含量最低。对6种激素含量进行相关性分析,发现不同嫁接组合接口处JA与SA含量呈极显著正相关,CTK与ABA含量呈极显著正相关;CTK与JA含量呈极显著负相关,ABA与JA含量呈显著负相关,SA与CTK含量呈显著负相关。【结论】番茄嫁接苗接口处的生理生化状态与嫁接亲和性有关,砧穗亲和性好,其接口处氧化应激程度低,亲和性越差的组合其氧化应激程度越高,且嫁接亲和性不仅体现在嫁接后能否正常生长,还体现在砧木与接穗嫁接后的共生过程。嫁接接口处的激素含量与砧�【Objective】The research aimed to study the affinity performance of solanaceous crops grafting,and explore the physiological and biochemical reactions at the interface of tomato scion grafted on different rootstocks.【Method】By setting tomato scion self-grafting(TS/TS)as the control,and comparing with tomato scion grafting tomato rootstock(TS/TR),tomato scion grafting eggplant rootstock(TS/ER),tomato scion grafting pepper rootstock(TS/PR),the activities of polyphenol oxidase(PPO),peroxidase(POD)and superoxide dismutase(SOD)at different time points after grafting of different grafting combinations were systematically evaluated.The contents of total phenols,total flavonoids,malondialdehyde(MDA),auxin(IAA),cytokinin(CTK),gibberellin(GA),abscisic acid(ABA),jasmonic acid(JA)and salicylic acid(SA)were determined.【Result】It was found that TS/TS,TS/TR and TS/ER had good affinity,while TS/PR had poor affinity.After 24 days of grafting,the total phenols,total flavonoids and MDA contents of TS/PR were 2.26 mg/g,1.88 mg/g and 6.51μmol/L,respectively,while those of TS/TS were 0.65 mg/g,0.26 mg/g and 2.66μmol/L,respectively.The contents of total phenols,total flavonoids and MDA at the interface of TS/TR and TS/ER with good self-grafting and affinity were significantly lower than those of TS/PR,the degree of oxidative stress at the interface was less.The total phenols,total flavonoids and MDA contents at the interface of TS/TR and TS/ER were significantly higher than those of TS/TS at the growth stage.PCA analysis of enzyme activity and related substance content at the interface showed that there were significant differences in interface oxidation related indexes between the same grafting and the different grafting.The determination of six hormones at the interface of different grafting combinations showed that CTK content was the lowest and JA content was the highest at the self-grafting interface,while CTK content was the highest and JA content was the lowest at the TS/PR combination interface.The correlation anal

关 键 词：嫁接 番茄 砧木 氧化酶 植物激素 亲和性 

分 类 号：S641.2[农业科学—蔬菜学]