水稻磷脂酶D基因家族鉴定及表达分析  

Identification and Expression Analysis of the Phospholipase D Gene Family in Rice

作　　者：曹华盛 李堂 熊亮 王福军[1] 李曙光[1] 顾海永[1] 何高[1] 梁世胡[1] 罗文永[1] CAO Huasheng;LI Tang;XIONG Liang;WANG Fujun;LI Shuguang;GU Haiyong;HE Gao;LIANG Shihu;LUO Wenyong(Rice Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of New Technology for Rice Breeding/Guangdong Rice Engineering Laboratory,Guangzhou 510640,China)

机构地区：[1]广东省农业科学院水稻研究所/广东省水稻育种新技术重点实验室/广东省水稻工程实验室,广东广州510640

出　　处：《广东农业科学》2024年第12期83-95,共13页Guangdong Agricultural Sciences

基　　金：广东省基础与应用基础研究基金(2021A1515010948);广东省重点领域研发计划(2020B202090003);广东省水稻育种新技术重点实验室项目(2023B1212060042)。

摘　　要：【目的】磷脂酶D(Phospholipase D,PLD)在植物生长发育、逆境胁迫响应等过程中起着至关重要的作用。挖掘参与水稻(Oryza sativa L.)生物和非生物胁迫的PLD基因家族成员,并探究其生物学功能。【方法】基于水稻基因组数据库鉴定水稻PLD家族成员,利用生物信息学方法系统分析其基因家族成员的蛋白理化性质、系统发育、保守基序、顺式作用元件,通过RNA-seq数据分析该家族成员在不同生物和非生物逆境胁迫条件下的表达谱,同时检测逆境处理条件下总磷脂酶D蛋白活性的变化。【结果】水稻PLD基因家族有17个成员,氨基酸数目在512~1115,相对分子质量在57.19~123.82 kD;蛋白等电点在5.60~11.23。根据系统进化树将水稻PLD基因家族分为4个亚家族,不同亚家族成员在外显子数量上存在显著差异;染色体定位分析发现,17个PLD基因定位在9条染色体上。顺式作用元件分析发现PLD基因家族成员启动子区域含有大量与生长素(IAA)、脱落酸(ABA)和茉莉酸(JA)等激素响应及低温响应、压力防御、厌氧诱导响应元件;生物和非生物逆境胁迫条件下RNA-seq数据分析发现,PLD基因家族成员受到低氮﹑ABA和赤霉素(GA)的强烈诱导;总磷脂酶D酶活分析显示高盐胁迫和黑条矮缩病侵染后的磷脂酶D总酶活显著升高。【结论】共鉴定出17个水稻PLD基因,同亚家族成员具有类似的保守基序。水稻PLD基因家族成员在不同组织及生物和非生物逆境条件下表达存在差异,这些结果将为解析磷脂酶D家族在水稻生长发育﹑生物和非生物逆境胁迫响应中的作用及其机制提供帮助。【Objective】Phospholipase D(PLD)plays a crucial role in plant growth,development,and stress response.The study was conducted to explore the PLD gene family members involved in biotic and abiotic stress in rice(Oryza sativa L.)and investigate their biological functions.【Method】The rice PLD family members were identified based on the rice genome database,and the protein physicochemical properties,phylogenetics,conserved motifs,and cis-acting elements of the gene family members were systematically analyzed by bioinformatics methods.The gene expression patterns of the family members under different biotic and abiotic stress conditions were analyzed through RNA-seq data,and the changes of total PLD activity under stress treatment conditions were detected.【Result】The rice PLD gene family had 17 members with amino acid numbers ranging from 512 to 1115 and relative molecular weights ranging from 57.19 to 123.82 kD;The isoelectric points of these proteins were between 5.60 and 11.23.According to the phylogenetic tree,the PLD gene family was divided into four subfamilies,and there were significant differences in the number of exons among the members of different subfamilies.Chromosome localization analysis revealed that 17 PLD genes were located on 9 chromosomes.The analysis of cis-acting elements revealed that the promoter regions of PLD gene family members contained a large number of hormone responsive elements such as auxin,abscisic acid,and methyl jasmonate,as well as low temperature response,pressure defense,and anaerobic induction responsive elements;RNA-seq data analysis under biotic and abiotic stress conditions revealed that members of the PLD gene family were strongly induced by low nitrogen,ABA,and GA;In addition,enzyme activity analysis showed a significant increase of total phospholipase D activity after high salt stress and black stripe dwarf disease infection.【Conclusion】A total of 17 rice PLD genes are identified,and members in the same subfamily have similar conserved motifs.The expression o

关 键 词：水稻 磷脂酶D 基因家族 生物信息学 系统发育分析 表达分析 

分 类 号：S511.032[农业科学—作物学]