构树GRAS基因家族鉴定及在茎发育中的表达分析  

Identification of GRAS Gene Family in Paper Mulberry and Analysis of Its Expression in Stem Development

作　　者：陈谭星 栗焕楠 王品胜 蒋亚君 肖进彬 闫灵敏 王洋 范毅 CHEN Tanxing;LI Huannan;WANG Pinsheng;JIANG Yajun;XIAO Jinbin;YAN Lingmin;WANG Yang;FAN Yi(Henan High-tech Industrial Co.,Ltd./Henan Broussonetia papyrifera Industrial Engineering Technology Research Center,Zhengzhou 450002,China;Henan Academy of Sciences,Zhengzhou 450002,China;Zhengzhou Zhongyuan District Urban Management Comprehensive Administrative Law Enforcement Brigade,Zhengzhou 450007,China;Xuchang Vocational and Technical College,Xuchang 461000,China)

机构地区：[1]河南省高新技术实业有限公司/河南省构树产业工程技术研究中心,河南郑州450002 [2]河南省科学院,河南郑州450002 [3]郑州市中原区城市管理综合行政执法大队,河南郑州450007 [4]许昌职业技术学院,河南许昌461000

出　　处：《广东农业科学》2024年第12期107-119,共13页Guangdong Agricultural Sciences

基　　金：河南省科学院基本科研业务费项目(240614033)。

摘　　要：【目的】GRAS转录因子在植物防御应答、根的发育、分生组织的形成、赤霉素(Gibberellin,GA)信号及光信号转导等过程中起重要调控作用,鉴定构树(Broussonetia papyrifera)中GRAS基因家族成员并分析其在茎组织中的表达模式,可为研究构树茎木质化发育提供参考。【方法】利用HMM在构树全基因组中鉴定GRAS家族基因,通过生物信息学方法及RNA-Seq数据分析GRAS基因在构树不同木质化程度茎组织(T1~T3)的表达模式。【结果】从构树基因组鉴定到43个GRAS成员,并按染色体分布进行命名。74%的BpGRAS无内含子,23%的BpGRAS仅含有1~2个内含子。多数BpGRAS蛋白为酸性氨基酸、不稳定蛋白、亲水蛋白,二级结构均以无规则卷曲和α螺旋为主。与拟南芥GRAS蛋白进行系统发育及蛋白保守结构域分析显示,43个构树GRAS成员被分为9类,且与拟南芥GRAS蛋白具有较高的同源性。顺式作用元件分析表明,该家族可能在植物光反应、逆境胁迫响应及植物体内源激素调控的相关生物学过程中具有潜在功能。基于RNA-Seq的表达分析可知,BpGRAS6、BpGRAS11在T1~T3中连续显著上调,BpGRAS1、BpGRAS15、BpGRAS20、BpGRAS23、BpGRAS29在T1~T3中显著下调。【结论】成功鉴定出43个构树GRAS基因家族成员,推测BpGRAS6、BpGRAS11、BpGRAS1、BpGRAS15、BpGRAS20、BpGRAS23、BpGRAS29可能在构树茎木质部形成方面发挥作用,可作为研究构树木质化调控机制的候选基因。【Objective】GRAS transcription factors play an important regulatory role in plant defense response,root development,meristem formation,gibberellin(GA)signaling and optical signal transduction.Members of the GRAS gene family in paper mulberry were identified and their expression patterns in stem tissues were analyzed,which could provide a reference for studying the lignification and development of paper mulberry stems.【Method】HMM was used to identify GRAS family genes in the whole genome of paper mulberry,and the expression patterns of GRAS genes in paper mulberry stem tissues(T1-T3)with different lignification degrees were analyzed through bioinformatics methods and RNA-Seq data.【Result】A total of 43 members of the GRAS family from the genome of paper mulberry was identified,and they were named according to their chromosome distribution.74%of BpGRAS had no introns,and 23%of BpGRAS contained only 1 to 2 introns.Most BpGRAS proteins were acidic amino acids,unstable proteins,and hydrophilic proteins.The secondary structure was dominated by irregular curls and alpha helices.Phylogenetic and protein conserved structure analysis were carried out together with Arabidopsis GRAS protein.The results showed that the 43 GRAS members of paper mulberry were divided into 9 categories and had high homology with Arabidopsis GRAS protein.The analysis of cis-acting elements showed that this family may have potential functions in the related biological processes of plant photoreaction,response to adversity stress,and regulation of plant-derived hormones.Expression analysis based on RNA-Seq showed that BpGRAS6 and BpGRAS11 were continuously significantly up-regulated in T1-T3,and BpGRAS1,BpGRAS15,BpGRAS20,BpGRAS23,and BpGRAS29 were continuously significantly down-regulated in T1-T3.【Conclusion】In this study,43 members of the GRAS gene family were identified from the genome of paper mulberry.It was hypothesized that BpGRAS6,BpGRAS11,BpGRAS1,BpGRAS15,BpGRAS20,BpGRAS23,and BpGRAS29 may play a role in the formation of the

关 键 词：构树 GRAS 转录因子 茎发育 木质素 生物信息学 

分 类 号：S792.99[农业科学—林木遗传育种]