转录组测序分析冷水游泳运动调节大鼠机体炎症反应的机制  

Transcriptome sequencing analysis of the mechanism by which cold water swimming regulates inflammatory response in rats

作　　者：司俊成 彭丽娜 孙莉莉 王宇 石磊 沈雯慧 李孟琪 臧万里 Si Juncheng;Peng Lina;Sun Lili;Wang Yu;Shi Lei;Shen Wenhui;Li Mengqi;Zang Wanli(Graduate School,Harbin Sport University,Harbin 150001,Heilongjiang Province,China;School of Sports Science and Health,Harbin Sport University,Harbin 150001,Heilongjiang Province,China)

机构地区：[1]哈尔滨体育学院研究生院,黑龙江省哈尔滨市150001 [2]哈尔滨体育学院运动科学与健康学院,黑龙江省哈尔滨市150001

出　　处：《中国组织工程研究》2025年第29期6205-6211,共7页Chinese Journal of Tissue Engineering Research

基　　金：哈尔滨体育学院实验室平台专项(LAB2021-07);项目负责人:彭丽娜。

摘　　要：背景:冷环境中运动,机体炎症反应受冷应激及运动应激双因素作用,其作用机制仍有待探究。目的:基于转录组测序技术探究冷水游泳对大鼠机体炎症反应的影响及机制。方法:40只雄性SD大鼠随机分为室温对照组、室温游泳组、冷水对照组、冷水游泳组,每组10只。室温对照组无干预,自由进食;室温游泳组游泳30 min/次,6次/周,共5周,水温(28±2)℃,水深35 cm;冷水对照组将大鼠放在水深3 cm的水箱中,水温(18±2)℃,自由活动;冷水游泳组游泳30 min/次,6次/周,共5周,水温(18±2)℃,水深35 cm。ELISA法检测各组大鼠血清白细胞介素6、肿瘤坏死因子α和高敏C-反应蛋白表达水平;基于转录组测序结果筛选差异表达基因绘制韦恩图和热图,并进行基因本体论和京都基因与基因组百科全书富集分析,蛋白互作网络筛选核心基因;RT-qPCR检测大鼠脾脏组织IRF7,OAS2,OASL mRNA表达情况。结果与结论:①ELISA检测结果显示,与室温对照组相比,室温游泳组和冷水游泳组各炎症指标水平显著升高(P<0.05),冷水对照组无显著差异;与室温游泳组相比,冷水游泳组各炎症指标表达无显著差异;与冷水对照组相比,冷水游泳组白细胞介素6和肿瘤坏死因子α表达呈上升趋势,高敏C-反应蛋白表达水平显著上升(P<0.05);②转录组分析结果显示,韦恩图显示受温度及运动干预双因素影响的差异表达基因共有39个;聚类热图分析结果表明,室温游泳组和冷水游泳组基因表达趋势整体相似,冷水对照组呈相反趋势;基因本体论和京都基因与基因组百科全书富集分析结果表明,差异表达基因富集于免疫系统、运动、核酸结合转录因子活性、NOD样受体信号通路等途径,NOD样受体信号通路富集基因数相对较多,且q value值较小,可能为关键通路;蛋白互作网络筛选出IRF7,OAS2,OASL,IFIT2,IFIT3等核心基因;③RT-qPCR验证结果显示,与室温对照组相比,室BACKGROUND:When exercising in a cold environment,the body’s inflammatory response is affected by both low temperature and exercise intervention,and its impact and mechanism remain to be explored.OBJECTIVE: To explore the effects and mechanisms of cold water swimming on inflammatory response of rats based on transcriptome sequencing technology. METHODS: 40 male SD rats were randomly divided into room temperature control group, room temperature swimming group, cold water control group, and cold water swimming group, with 10 rats in each group. The room temperature control group had no intervention and was free to eat. The room temperature swimming group received swimming at 30 min/time, 6 times/week, for 5 weeks;the water temperature was (28±2) °C, and the water depth was 35 cm. In the cold water control group, the rats were placed in a water tank with a depth of 3 cm;the water temperature was (18±2) °C, and they were free to move. The cold water swimming group received swimming at 30 min/time, 6 times/week, for 5 weeks;the water temperature was (18±2) °C, and the water depth was 35 cm. Enzyme-linked immunosorbent assay was used to detect the levels of serum interleukin-6, tumor necrosis factor-α, and high-sensitivity C-reactive protein. Based on the transcriptome sequencing results, differentially expressed genes were screened to draw Venn diagrams and heat maps, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were performed. The protein-protein interaction network was used to screen core genes. RT-qPCR was used to detect the mRNA expression of IRF7, OAS2, and OASL in rat spleen tissue. RESULTS AND CONCLUSION: (1) The ELISA results showed that compared with the room temperature control group, the levels of various inflammatory indicators in the room temperature swimming group and the cold water swimming group were significantly increased (P < 0.05), and there was no significant difference in the cold water control group. Compared with the room temperature swimming group,

关 键 词：冷水游泳 炎症反应 转录组 NOD样受体信号通路 差异表达基因 工程化组织构建 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R875