督脉电针抑制铁死亡促进急性颈髓损伤模型大鼠的神经修复  

Du Meridian electroacupuncture inhibits ferroptosis and promotes neurorepair in rats with acute cervical spinal cord injury

作　　者：孙荣妍 许卢春 姜国正 宋佳伟 马昱堃 范娇娇 王冠龙 杨永栋[2] 俞兴[2] Sun Rongyan;Xu Luchun;Jiang Guozheng;Song Jiawei;Ma Yukun;Fan Jiaojiao;Wang Guanlong;Yang Yongdong;Yu Xing(Haidian Hospital of Beijing,Beijing 100080,China;Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京市海淀医院,北京市100080 [2]北京中医药大学东直门医院,北京市100700

出　　处：《中国组织工程研究》2025年第29期6228-6236,共9页Chinese Journal of Tissue Engineering Research

基　　金：中央高校基本科研业务费(2024-JYB-JBZD-065),项目负责人:杨永栋;国家自然科学基金面上项目(81973882),项目负责人:俞兴;国家自然科学基金青年项目(81804119),项目负责人:杨永栋;北京中医药大学东直门医院人才培养计划(DZMG-QNGG0010),项目负责人:杨永栋。

摘　　要：背景:近年来,多项研究证实督脉电针在减轻脊髓损伤方面有独特的效果,需进一步明确相关机制。目的:探讨督脉电针对大鼠颈髓损伤后铁死亡的调节作用及作用机制。方法:将100只SD大鼠随机分为假手术组、模型组、督脉电针组、RSL3组和督脉电针+RSL3组。假手术组仅切除椎板,其他4组通过Allen法构建急性颈髓损伤模型,督脉电针组在颈髓损伤后给予电针治疗,RSL3组在颈髓损伤后接受谷胱甘肽过氧化物酶4抑制剂RSL3腹腔注射,督脉电针+RSL3组在颈髓损伤后予以督脉电针以及RSL3腹腔注射。术后7,28 d取材,观察大鼠运动功能、组织学形态、神经元存活、胶质瘢痕形成、氧化应激水平、Fe^(2+)含量以及谷胱甘肽过氧化物酶4和长链酰基辅酶A合成酶4表达。结果与结论:①最终90只大鼠完成后续实验,每组18只;②督脉电针组大鼠的FLS评分和BBB评分显著高于模型组和督脉电针+RSL3组(P<0.05);③与模型组相比,督脉电针组可以改善颈髓组织学形态和线粒体微观形态,而RSL3逆转了督脉电针对线粒体的保护作用;④与模型组相比,督脉电针组微管相关蛋白2、谷胱甘肽过氧化物酶4、谷胱甘肽和超氧化物歧化酶表达显著增加(P<0.05),胶质纤维酸性蛋白、长链酰基辅酶A合成酶4、活性氧、丙二醛和Fe^(2+)含量显著减少(P<0.05),而RSL3逆转了督脉电针对铁死亡、脂质过氧化和氧化应激的抑制作用;⑤结果表明,督脉电针可以通过调控谷胱甘肽过氧化物酶4/长链酰基辅酶A合成酶4轴抑制铁死亡,从而减少颈髓损伤后神经元继发性损伤及胶质瘢痕形成,改善神经功能。BACKGROUND:Recent studies have shown that Du Meridian electroacupuncture has a unique effect on alleviating spinal cord injury,but the underlying mechanisms require further clarification.OBJECTIVE:To investigate the regulatory effects and the associated action mechanisms of Du Meridian electroacupuncture on ferroptosis after cervical spinal cord injury in rats.METHODS:One hundred SD rats were randomly divided into sham,model,Du Meridian electroacupuncture,RSL3,and Du Meridian electroacupuncture+RSL3 groups.The sham group underwent only laminectomy.The other four groups were subjected to cervical spinal cord injury by the Allen method.The Du Meridian electroacupuncture group received electroacupuncture after cervical spinal cord injury.The RSL3 group received intraperitoneal injections of glutathione peroxidase 4 inhibitor RSL3 after cervical spinal cord injury.The Du Meridian electroacupuncture+RSL3 group received both electroacupuncture and RSL3 intervention after cervical spinal cord injury.Samples were collected on postoperative days 7 and 28 to assess motor function,histological morphology,neuronal survival,glial scar formation,oxidative stress levels,Fe^(2+)content,glutathione peroxidase 4,and long-chain acyl-CoA synthetase 4 expression.RESULTS AND CONCLUSION:(1)Finally,90 rats completed the follow-up experiment,with 18 rats in each group.(2)FLS and BBB scores were significantly higher in the Du Meridian electroacupuncture group compared with the model and Du Meridian electroacupuncture+RSL3 groups(P<0.05).(3)Compared with the model group,Du Meridian electroacupuncture improved cervical spinal cord tissue morphology and mitochondrial ultrastructure,while these effects were inhibited by RSL3.(4)Du Meridian electroacupuncture increased the expression of microtubule-associated protein 2,glutathione peroxidase 4,glutathione,and superoxide dismutase(P<0.05)and reduced the expression of glial fibrillary acidic protein,long-chain acyl-CoA synthetase 4,reactive oxygen species,malondialdehyde,and Fe^(2+)compared with

关 键 词：颈髓损伤 督脉电针 铁死亡 谷胱甘肽过氧化物酶4 长链酰基辅酶A合成酶4 氧化应激 神经修复 工程化组织构建 

分 类 号：R496[医药卫生—康复医学] R-332[医药卫生—临床医学] R245