脾虚型高脂血症模型小鼠的构建与评价  

Construction and evaluation of spleen-deficiency hyperlipidemia mouse models

作　　者：陈丽娟[1,2] 高心雪 吴瑾[1,2] 杜莹[1,2] 吕美君[1,2] 隋国媛 贾连群[1] 潘国伟 Chen Lijuan;Gao Xinxue;Wu Jin;Du Ying;Lyu Meijun;Sui Guoyuan;Jia Lianqun;Pan Guowei(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning Province,China;Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning Province,China;China Medical University,Shenyang 110122,Liaoning Province,China)

机构地区：[1]辽宁中医药大学,辽宁省沈阳市110847 [2]辽宁中医药大学中医脏象理论及应用教育部重点实验室,辽宁省沈阳市110847 [3]中国医科大学,辽宁省沈阳市110122

出　　处：《中国组织工程研究》2025年第29期6237-6242,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(82204949),项目负责人:陈丽娟;辽宁省科技计划联合计划(基金)项目(2023-MSLH-192),项目负责人:陈丽娟;辽宁省教育厅面上项目(JYTMS20231813),项目负责人:陈丽娟;辽宁省教育厅高校基本科研项目储备项目(2024-JYTCB-013),项目负责人:陈丽娟;辽宁中医药大学中医脏象理论及应用教育部重点实验室开放基金项目(zyzx2008),项目负责人:陈丽娟。

摘　　要：背景:中医药防治脾虚高脂血症具有独特优势,基础研究中的脾虚高脂血症造模动物主要有大鼠、猪等,这对只能应用小鼠模型的医学研究而言具有局限性,亟待建立并评价脾虚高脂血症小鼠模型以支持中医药防治脾虚高脂血症的基础研究。目的:建立脾虚高脂血症小鼠模型。方法:采用随机数字表法将24只C57BL/6J小鼠随机分为正常组(n=12)和脾虚高脂组(n=12),正常组给予正常饲料喂养;脾虚高脂组采用饮食失常+劳倦内伤+高脂饲料喂养的方法制备脾虚高脂模型,前2周,单日游泳到耐力极限且仅喂食甘蓝,自由饮水,双日灌胃炼制猪油+高脂饲料喂养,2周后每天以高脂饲料喂养,持续至12周。高脂饲料喂养4,12周,检测小鼠体质量、饮食量、抓力、粪便含水量、小肠炭末推进率、血清D-木糖与胃泌素水平、脾脏指数与胸腺指数、血脂水平、全身脂肪质量、体脂率、肝脏脂质沉积。结果与结论:①与正常组相比,脾虚高脂组小鼠高脂饲料喂养4,12周的体质量、粪便含水量、全身脂肪质量、体脂率、三酰甘油与总胆固醇水平均升高(P<0.05),高脂饲料喂养4,12周的日饮食量、抓力、D-木糖水平均降低(P<0.05),高脂饲料喂养4周的脾脏指数升高(P<0.05),高脂饲料喂养12周的小肠炭末推进率、胃泌素水平、脾脏指数、胸腺指数均降低(P<0.05),高脂饲料喂养12周的低密度脂蛋白胆固醇水平升高(P<0.05);②肝脏油红O染色结果显示,脾虚高脂组高脂饲料喂养4周的脂质沉积稍多于正常组,高脂饲料喂养12周的脂质沉积明显多于正常组;③结果表明,采用饮食失常+劳倦内伤+高脂饲料喂养的方法可制备出稳定的脾虚高脂血症小鼠模型。BACKGROUND:Traditional Chinese medicine has unique advantages in preventing and treating spleen-deficiency and hyperlipidemia.In basic studies,models of spleen-deficiency and hyperlipidemia are commonly found in rats,pigs,and other animals.This has limitations for medical research that can only use mouse models.It is urgent to establish and evaluate mouse models of spleen-deficiency and hyperlipidemia to support basic research on traditional Chinese medicine in preventing and treating spleen-deficiency and hyperlipidemia.OBJECTIVE:To establish a mouse model of spleen-deficiency hyperlipidemia.METHODS:Totally 24 C57BL/6J mice were randomly divided into normal group(n=12)and spleen-deficiency hyperlipemia group(n=12).Mice in normal group were fed basic diet.Mice in the spleen-deficiency hyperlipemia group were prepared with a diet disorder+fatigue internal injury+high-fat feeding method to establish a spleen-deficiency high-fat model.In the first 2 weeks,the mice were forced to swim to their endurance limit on a single day and were only fed cabbage,with free access to water.They were also gavaged with refined lard+high-fat feed on two-day intervals.After 2 weeks,the mice were fed a high-fat diet every day and the diet continued until 12 weeks.The mice were fed with a high-fat diet for 4 and 12 weeks,and their body weight,food intake,gripping strength,fecal water content,small intestinal charcoal propulsion rate,serum D-xylose and gastrin levels,spleen index and thymus index,blood lipid level,total body fat mass,body fat percentage,and liver lipid deposition were tested.RESULTS AND CONCLUSION:(1)Compared with the normal group,the body weight,fecal water content,total body fat mass,body fat percentage,triglyceride and total cholesterol levels of the mice in the spleen-deficiency hyperlipemia group fed with high-fat diet for 4 and 12 weeks were increased(P<0.05);the daily food intake,gripping force,and D-xylose level of the mice fed with high-fat diet for 4 and 12 weeks were decreased(P<0.05);the spleen index of the m

关 键 词：脾虚 高脂血症 小鼠 动物模型 模型评价 工程化组织构建 

分 类 号：R459.9[医药卫生—治疗学] R319[医药卫生—临床医学] R259