运动对抑郁大鼠小胶质细胞和星形胶质细胞激活及神经元凋亡的影响  

Effects of exercise on activation of microglia and astrocytes and neuronal apoptosis in depressed rats

作　　者：杜鹃[1] 张毅 郝泉水 Du Juan;Zhang Yi;Hao Quanshui(School of Physical Education,Jianghan University,Wuhan 430056,Hubei Province,China;Huanggang Central Hospital Affiliated to Yangtze University,Huanggang 438000,Hubei Province,China)

机构地区：[1]江汉大学体育学院,湖北省武汉市430056 [2]长江大学附属黄冈市中心医院,湖北省黄冈市438000

出　　处：《中国组织工程研究》2025年第29期6243-6248,共6页Chinese Journal of Tissue Engineering Research

基　　金：湖北省卫生健康委2021-2022年度科研项目(WJ2021M084);项目负责人:郝泉水。

摘　　要：背景:环加氧酶2可显著增强大鼠抑郁样行为,环加氧酶2抑制剂具有抗抑郁作用。运动可明显减少抑郁患者的抑郁样行为,然而,目前尚不清楚运动是否通过抑制环加氧酶2来发挥抗抑郁作用。目的:旨在揭示运动对抑郁大鼠环加氧酶2表达、小胶质细胞和星形胶质细胞激活、氧化应激和神经元凋亡的影响,以及可能的分子机制。方法:通过慢性不可预测的温和刺激诱导抑郁症大鼠模型,将建模成功的大鼠随机分为刺激组和转轮运动组,每组10只;另外选择10只正常大鼠作为对照组。对照组和刺激组大鼠不运动;转轮运动组大鼠进行强制转轮运动,共运动4周。通过蔗糖偏爱测试评价抑郁样行为,免疫荧光染色检测海马环加氧酶2表达,蛋白质印迹分析检测海马环加氧酶2、Iba-1、CD11b、CD45、胶质纤维酸性蛋白、Bcl-2、Bax和cleaved caspase-3蛋白表达,RT-PCR检测海马环加氧酶2 mRNA水平,使用试剂盒测定海马组织匀浆中相关抗氧化酶的活性。结果与结论:与刺激组相比,转轮运动组的蔗糖消耗量增加,海马内的环加氧酶2表达降低,海马内的超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶水平增加,丙二醛水平降低,海马内的Iba1、CD11b和CD45蛋白表达降低,胶质纤维酸性蛋白水平降低,Bcl-2的蛋白表达升高,Bax和cleaved caspase-3蛋白表达降低(P<0.05)。研究表明:转轮运动可通过抑制慢性不可预测温和刺激大鼠海马中环加氧酶2的表达来减少抑郁样行为,并通过抑制海马中小胶质细胞和星形胶质细胞激活、氧化应激和神经元凋亡来发挥抗抑郁作用。BACKGROUND:Cyclooxygenase-2 can significantly enhance depressive-like behavior in rats,and cyclooxygenase-2 inhibitors have antidepressant effects.Exercise can significantly reduce depressive-like behaviors in depressed patients.However,it is currently unclear whether exercise exerts an antidepressant effect by inhibiting cyclooxygenase-2.OBJECTIVE:To reveal the effects of exercise on cyclooxygenase-2 expression,microglia and astrocyte activation,oxidative stress and neuronal apoptosis in depressed rats,as well as the possible molecular mechanisms.METHODS:The rat model of depression was induced by chronic unpredictable mild stimulation.After modeling,the chronic unpredictable mild stimulation rats were randomly divided into stimulation group and wheel running group,with 10 rats in each group.In addition,10 normal rats were selected as the control group.The rats in the control group and stimulation group did not exercise.The rats in the wheel running group performed forced wheel running for a total of 4 weeks.Depressive-like behavior was evaluated by sucrose preference test.Hippocampal cyclooxygenase-2 expression was detected by immunofluorescence staining.The expression of cyclooxygenase-2,Iba-1,CD11b,CD45,glial fibrillary acid protein,Bcl-2,Bax,and cleaved caspase-3 proteins in the hippocampus was detected by western blot analysis.The cyclooxygenase-2 mRNA level in the hippocampus was detected by RT-PCR.The activities of antioxidant enzymes in hippocampal tissue homogenates were determined using kits.RESULTS AND CONCLUSION:Compared with stimulation group,sucrose consumption was increased;cyclooxygenase-2 expression in hippocampus was decreased;superoxide dismutase,catalase,and glutathione peroxidase levels in hippocampus were increased;malonaldehyde level was decreased;Iba1,CD11b,and CD45 protein expression levels in hippocampus were decreased,and glial fibrillary acid protein level in hippocampus was decreased.Bcl-2 protein expression levels in the hippocampus were increased,while Bax and cleaved caspase-3 leve

关 键 词：抑郁 转轮运动 CUMS 环加氧酶2 氧化应激 神经元凋亡 小胶质细胞 星形胶质细胞 工程化组织构建 

分 类 号：R496[医药卫生—康复医学] R318[医药卫生—临床医学] R749.4