血流限制抗阻运动对肥胖青年男性血清代谢物的急性影响  

Acute effects of blood flow restriction resistance exercise on serum metabolites in obese young men

作　　者：刘昊为 田浩冬 黄丽 余杭林 彭莉[1] Liu Haowei;Tian Haodong;Huang Li;Yu Hanglin;Peng Li(School of Physical Education,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学体育学院,重庆市400715

出　　处：《中国组织工程研究》2025年第29期6249-6259,共11页Chinese Journal of Tissue Engineering Research

基　　金：国家社会科学基金项目(21BTY092),项目负责人:彭莉;重庆市博士研究生科研创新项目(CYB240087),项目负责人:田浩冬。

摘　　要：背景:血流限制抗阻运动是一种新兴的抗阻运动方式,在较低负荷下限制血流供应,能获得与高负荷运动相似甚至更好的效果。目前关于血流限制抗阻运动的具体作用机制及在肥胖改善方面的应用研究仍较为有限。探索血流限制抗阻运动的潜在机制及其在肥胖人群中的应用,有助于为肥胖人群提供全新的运动处方选择。目的:通过比较单次传统抗阻运动与血流限制抗阻运动对肥胖青年男性血清代谢物的影响差异,探讨血流限制抗阻运动对肥胖相关代谢影响的潜在机制。方法:对25名肥胖青年男性进行单次传统抗阻运动(n=13)或血流限制抗阻运动(n=12)干预。在运动前、运动后即刻、运动后1 h和运动后24 h采集静脉血样本,采用高效液相色谱串联质谱联用(LC-MS/MS)技术检测血清代谢组,对三大能源物质(糖类、脂类和氨基酸类)代谢物及相关代谢通路进行分析。结果与结论:①传统抗阻运动和血流限制抗阻运动均能诱导血清代谢谱的急性改变。②对于传统抗阻运动组,发现2种糖类差异代谢物,4种脂类差异代谢物和3种氨基酸类差异代谢物;对于血流限制抗阻运动组,发现10种脂类差异代谢物,3种氨基酸类差异代谢物。③传统抗阻运动和血流限制抗阻运动均能显著影响肥胖男青年的血清代谢物,且在运动后1 h内达到最大影响。相比之下,血流限制抗阻运动对代谢物的影响更加广泛且持续时间更长。④传统抗阻运动在糖类代谢物调控上更为显著,尤其是D-半乳糖和甘油的消耗,而血流限制抗阻运动在脂类和氨基酸代谢物的调控中表现出独特优势,如磷脂类的增加及天门冬氨酸对脂肪酸氧化的潜在促进作用。这些差异可能与炎症调节和胰岛素敏感性相关,但其临床意义尚需进一步研究验证。BACKGROUND:Blood flow restriction resistance exercise is an emerging mode of resistance exercise that restricts blood flow at lower loads,achieving effects similar to or even better than high-load exercises.Current research on its specific mechanisms and applications in obesity improvement is still limited.Exploring the potential mechanisms of blood flow restriction resistance exercise and its application in obese populations could provide a novel exercise prescription option for this group.OBJECTIVE:To explore the potential mechanisms of blood flow restriction resistance exercise on obesity-related metabolism by comparing the effect of a single session of traditional resistance exercise and blood flow restriction resistance exercise on serum metabolites in obese young men.METHODS:Twenty-five obese young men underwent a single session of traditional resistance exercise(n=13)or blood flow restriction resistance exercise(n=12).Venous blood samples were collected before exercise,immediately post-exercise,1 hour post-exercise,and 24 hours post-exercise.High-performance liquid chromatography-tandem mass spectrometry was used to analyze the serum metabolome,focusing on the metabolites of the three major energy substances(carbohydrates,lipids,and amino acids)and related metabolic pathways.RESULTS AND CONCLUSION:(1)Both resistance exercise and blood flow restriction resistance exercise induced acute changes in the serum metabolomic profile.(2)In the traditional resistance exercise group,two carbohydrate differential metabolites,four lipid differential metabolites,and three amino acid differential metabolites were identified.In the blood flow restriction resistance exercise group,10 lipid differential metabolites and 3 amino acid differential metabolites were discovered.(3)Both traditional resistance exercise and blood flow restriction resistance exercise significantly affected the serum metabolites in obese young men,with the greatest impact observed 1 hour post-exercise.Comparatively,blood flow restriction resistance ex

关 键 词：血流限制抗阻运动 肥胖 血清代谢物 代谢组学 糖代谢 脂代谢 氨基酸代谢 工程化组织构建 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] G804.23[文化科学—运动人体科学]