Col003对血管紧张素Ⅱ诱导的血管平滑肌细胞增殖和迁移的影响  

Effect of Col003 on proliferation and migration of vascular smooth muscle cells induced by angiotensin Ⅱ

作　　者：邓梦雨 谢晓云 周克剑 谢育良 刘竞丽[1] DENG Mengyu;XIE Xiaoyun;ZHOU Kejian;XIE Yuliang;LIU Jingli(Department of Neurology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China)

机构地区：[1]广西医科大学第一附属医院神经内科,广西南宁市530021

出　　处：《广西医学》2025年第1期72-76,共5页Guangxi Medical Journal

基　　金：国家自然科学基金(8216050094);广西自然科学基金(2023GXNSFDA026017)。

摘　　要：目的探讨Col003对血管紧张素Ⅱ(AngⅡ)诱导的血管平滑肌细胞(VSMCs)增殖和迁移的影响。方法(1)体外培养VSMCs,给予不同浓度(25μmol/L、37.5μmol/L、50μmol/L、62.5μmol/L、75μmol/L)Col003处理后,采用乳酸脱氢酶法检测细胞毒性,筛选Col003的干预浓度。(2)体外培养VSMCs,给予不同浓度(0.01μmol/L、0.1μmol/L、1μmol/L、10μmol/L)AngⅡ处理后,采用CCK-8法检测细胞增殖活性,筛选AngⅡ诱导VSMCs增殖的最佳浓度。(3)将VSMCs分为空白对照组(给予细胞培养基处理)、AngⅡ组(给予1μmol/L AngⅡ处理)、AngⅡ+溶剂对照组(给予1μmol/L AngⅡ+二甲基亚砜处理)、AngⅡ+25μmol/L Col003组(给予1μmol/L AngⅡ+25μmol/L Col003处理)、AngⅡ+37.5μmol/L Col003组(给予1μmol/L AngⅡ+37.5μmol/L Col003处理)、AngⅡ+阿托伐他汀组(给予1μmol/L AngⅡ+阿托伐他汀处理),分别采用CCK-8法和划痕试验评估Col003对AngⅡ诱导的VSMCs增殖和迁移的影响。结果筛选出Col003干预浓度为25μmol/L和37.5μmol/L,AngⅡ干预浓度为1μmol/L。与AngⅡ组相比,AngⅡ+25μmol/L Co1003组、AngⅡ+37.5μmol/L Co1003组、AngⅡ+阿托伐他汀组的细胞存活率降低(P<0.05),AngⅡ+37.5μmol/LCo1003组、AngⅡ+阿托伐他汀组的划痕汇合率降低(P<0.05)。与AngⅡ+37.5μmol/L Col003组相比,AngⅡ+阿托伐他汀组的细胞存活率和划痕汇合率差异无统计学意义(P>0.05)。结论Col003可以抑制AngⅡ诱导的VSMCs增殖与迁移,可能具有潜在的抗动脉粥样硬化作用。Objective To explore the effect of Col003 on proliferation and migration of vascular smooth muscle cells(VSMCs)induced by angiotensin Ⅱ(AngⅡ).Methods(1)VSMCs were cultured in vitro,after different concentrations of Col003(25μmol/L,37.5μmol/L,50μmol/L,62.5μmol/L,75μmol/L)treatment,cytotoxicity was detected by employing the lactate dehydrogenase method for screening intervention concentration of Col003.(2)VSMCs were cultured in vitro,after different concentrations of AngⅡ(0.01μmol/L,0.1μmol/L,1μmol/L,10μmol/L)treatment,cell proliferation activity was detected by using the CCK-8 method for screening the optimal concentration of VSMCs proliferation induced by AngⅡ.(3)VSMCs were assigned to blank control group(receiving cell culture medium for treatment),AngⅡ group(receiving 1μmol/L AngⅡ for treatment),AngⅡ+solution control group(receiving 1μmol/L AngⅡ+dimethyl sulfoxide for treatment),AngⅡ+25μmol/L Col003 group(receiving 1μmol/L AngⅡ+25μmol/L Col003 for treatment),AngⅡ+37.5μmol/L Col003 group(receiving 1μmol/L AngⅡ+37.5μmol/L Col003 for treatment),or AngⅡ+atorvastatin group(receiving 1μmol/L AngⅡ+atorvastatin for treatment).The effect of Col003 on AngⅡ-induced VSMCs proliferation and migration was evaluate by employing the CCK-8 method and scratch test.Results The intervention concentrations of Col003 screened were 25μmol/L and 37.5μmol/L,and of AngⅡ screened was 1μmol/L.Compared with the AngⅡ group,the AngⅡ+25μmol/L Co1003 group,the AngⅡ+37.5μmol/L Co1003 group,and the AngⅡ+atorvastatin group exhibited a decreased cell survival rate(P<0.05);furthermore,the AngⅡ+37.5μmol/L Co1003 group and the AngⅡ+atorvastatin group yielded decreased scratch convergence rate(P<0.05).Compared with the AngⅡ+37.5μmol/L Col003 group,there was no statistically significant difference in cell survival rate and scratch convergence rate of the AngⅡ+atorvastatin group(P>0.05).Conclusion Col003 can inhibit the proliferation and migration of AngⅡ-induced VSMCs,which may

关 键 词：动脉粥样硬化 Col003 血管紧张素Ⅱ 血管平滑肌细胞 细胞实验 

分 类 号：R543.5[医药卫生—心血管疾病]