血液循环中12种血小板白细胞聚集体百分比和荧光强度比率参考区间的初步建立  

Preliminary establishment of reference intervals for percentage and fluorescence intensity ratios of 12 platelet-leukocyte aggregates in circulation

作　　者：代腾龙 梁爽 李斌 梁翠莹 岳欣阳 张海月 吴俊[1,2] DAI Tenglong;LIANG Shuang;LI Bin;LIANG Cuiying;YUE Xinyang;ZHANG Haijyue;WU Jun(Fourth Clinical School of Peking University,Beijing 102208;Medical Laboratory Center,Beijing Jishuitan Hospital Affiliated to Capital Medical University,Beijing 100035,Chin;Intensive Care Unit,Beijing Jishuitan Hospital Affiliated to Capital Medical University,Beijing 100035,China)

机构地区：[1]北京大学第四临床医学院,北京102208 [2]首都医科大学附属北京积水潭医院检验科,北京100035 [3]首都医科大学附属北京积水潭医院重症医学科,北京100035

出　　处：《临床检验杂志》2025年第2期130-135,共6页Chinese Journal of Clinical Laboratory Science

基　　金：北京市自然科学基金-大兴创新联合基金(L246006);首都医科大学附属北京积水潭医院自然基金培育项目(ZR-202412)。

摘　　要：目的初步建立血液循环中12种血小板白细胞聚集体百分比和荧光强度比率的参考区间。方法选取北京积水潭医院124例18~90岁的健康体检者,包括61名男性,63名女性。检测样本为研究对象静脉EDTA-K_(2)抗凝血,采用流式细胞术检测12种血小板白细胞聚集体的百分比和荧光强度比率,按性别分组进行比较分析。按WS/T402一2024标准计算各参数参考区间。结果男性血小板T淋巴细胞聚集体、血小板辅助T淋巴细胞聚集体和血小板细胞毒性T淋巴细胞聚集体百分比低于女性(P均<0.05)。男性血小板T淋巴细胞聚集体和血小板细胞毒性T淋巴细胞聚集体,血小板B淋巴细胞聚集体以及血小板NK细胞聚集体的荧光强度比率大于女性(P均<0.05)。结论初步建立了健康成人血液循环中12种血小板白细胞聚集体百分比和荧光强度比率的参考区间。性别可能会影响健康成人血小板白细胞聚集体尤其是血小板淋巴细胞类聚集体百分比和荧光强度比率检测结果,需要大样本量研究进一步证实。Objective To establish the preliminary reference intervals for the percentage and fluorescence intensity ratio(FIR)for 12 platelet-leukocyte aggregates(PLAs)in peripheral blood circulation.Methods A total of 124 healthy individuals(61 males and 63 females)aged 18-90 years were selected from Beijing Jishuitan Hospital.Venous blood samples were collected in EDTA-K_(2),tubes for anticoagulation,and flow cytometry was used to measure the percentage and FIR of 12 types of PLAs.All the participants were grouped by gender for comparative analysis.Reference intervals for each parameter were calculated according to the WS/T 4022024 standard.Results The percentages of platelet-T lymphocyte aggregates,platelet-CD4^(+)T lymphocyte aggregates,and platelet-CD8^(+)T lymphocyte aggregates in males were significantly lowerthan those in females(all P<0.05).The FIRs of platelet-Tlymphocyte aggregates,platelet CD8^(+)T lymphocyte aggregates,platelet-B lymphocyte aggregates,and platelet-NK cell aggregates in males were significantly higher than those in females(all P<0.05).Conclusion The preliminary reference intervals for the percentage and FIR of 12 types of PLAs in healthy adults have been established.Dfferent gender may influence the detection results of platelet-leukocyte aggregates,especially platelet-lymphocyte aggregates,and the corresponding FIR values in healthy adults.Further large-scale studies should be necessary to confirm these findings.

关 键 词：血小板白细胞聚集体 参考区间 流式细胞术 

分 类 号：R446[医药卫生—诊断学]