产肠毒素型大肠杆菌感染小鼠肠道菌群变化的分析  

Impact of Enterotoxigenic Escherichia Coli Infection on Gut Flora in Mice

作　　者：陈傲 王超 刘欣 李旭峰 李丽阳[1] Chen Ao;Wang Chao;Liu Xin;Li Xufeng;Li Liyang(College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2025年第1期80-87,共8页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省大学生创新创业训练计划项目(202110223034);黑龙江八一农垦大学横向科研项目(2023013)。

摘　　要：为分析产肠毒素型大肠杆菌(Enterotoxigenic Escherichia coli,ETEC)感染对小鼠肠道菌群的影响,选取若干ICR小鼠随机分为试验组和对照组,试验组采取ETEC-K88饲喂,而对照组给予等量PBS;48 h后将小鼠麻醉处死解剖取空肠内容物,进行16S r RNA测序及生物信息学分析。结果表明:ETEC-K88诱导小鼠发生腹泻后,菌群物种丰富度和多样性均受其影响。综上,ETEC-K88感染后显著影响小鼠肠道菌群物种多样性,小鼠肠道菌群的组成发生显著变化,为产肠毒素型大肠杆菌感染机理的研究提供了相关科学依据。To analyze the impact of the ETEC infection on gut flora of mice,a number of ICR mice were randomly divided into an experimental group attacked by ETEC-K88 and a PBS group as a control,and samples were dissected at the end of feeding for 16S r RNA sequencing and bioinformatics analysis.The results showed that ETEC-K88 induced diarrhea in mice,and the species richness and diversity of the bacterial flora were affected by it.In conclusion,the ETEC-K88 infection significantly affected the species diversity of the intestinal flora of mice,and the composition of the intestinal flora of mice changed significantly,which provided a relevant scientific basis for the study of the mechanism of enterotoxin-producing E.coli infection.

关 键 词：产肠毒素型大肠杆菌 肠道菌群 16S r RNA 

分 类 号：S852.6[农业科学—基础兽医学]