低温耐盐碱纤维素降解菌Streptomyces microflavus CLSD-1的分离鉴定与降解条件优化  

Isolation,Identification and Optimization of Degradation Conditions of Streptomyces mic-roflavus CLSD-1,a Low-temperature Saline-resistant Cellulose Degrading Bacterium

作　　者：刘雨欣 王敬红 张伟 洪艳华[3] 孙冬梅[1] 魏丹[4] 王伟东 Liu Yuxin;Wang Jinghong;Zhang Wei;Hong Yanhua;Sun Dongmei;Wei Dan;Wang Weidong(Northeast Plain Agricultural Green Low Carbon Key Laboratory of the Ministry of Agriculture and Rural Affairs/College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing163319;College of Life Science,Northeast Forestry University;College of Agronomy,Heilongjiang Bayi Agricultural University;Institute of Plant Nutrition,Resources and Environment,Beijing Academy of Agriculture and Forestry Sciences)

机构地区：[1]农业农村部东北平原农业绿色低碳重点实验室/黑龙江八一农垦大学生命科学技术学院,大庆163319 [2]东北林业大学生命科学学院 [3]黑龙江八一农垦大学农学院 [4]北京农林科学院植物营养与资源环境研究所

出　　处：《黑龙江八一农垦大学学报》2025年第1期96-104,共9页journal of heilongjiang bayi agricultural university

基　　金：国家自然科学基金(U22A20444,32170132);黑龙江省揭榜挂帅科技攻关项目(2022ZXJ08B01);中国科学院战略性先导科技专项(XDA28030203-2);黑龙江八一农垦大学三纵计划基金(ZDZX202105)。

摘　　要：寒区盐碱地纤维素微生物降解困难。获得低温耐盐碱纤维素降解菌株,提高其降解能力。从盐碱地采集土壤样品,以限制性培养筛选低温耐盐碱纤维素降解菌株,并对目标菌株进行纤维素降解能力的优化,以Plackett-Burman试验筛选得到的最佳响应因素为基础,通过最陡爬坡试验和响应面法优化菌株降解纤维素的最佳条件。分离得到1株可在15℃,3%NaCl和pH9.0条件下生长并具纤维素降解能力的菌株,经菌体形态观察、生理生化试验和16S r RNA测序分析,初步归类为细黄链霉菌(Streptomyces microflavus),命名为CLSD-1。其在初始条件下,滤纸酶活最大为84.25 U·L^(-1),培养至第15天滤纸降解率为20.21%,经优化后的最佳滤纸降解培养条件为氯化钙0.246 2 g·L^(-1)、装液量27%、转速169 rpm,其滤纸降解率达33.25%,相比优化前提高64.52%。细黄链霉菌CLSD-1可作为潜在的低温耐盐碱纤维素降解微生物资源。Microbial degradation of cellulose is difficult in saline-alkali soil in cold region.The low temperature saline-alkali resistant cellulose degrading strain was obtained and its degrading ability was improved.Soil samples were collected from saline-alkali soil,and the low temperature saline-alkali cellulose degrading strains were screened by restricted culture,and the cellulose degrading ability of the target strains was optimized.Based on the best response factors screened by Plackett-Burman test,the optimal conditions for cellulose degradation were optimized by the steepest climb test and response surface method.A cellulose degrading strain that could grow at 15℃,3%NaCl and pH9.0 was isolated and classified as Streptomyces microflavus(CLSD-1)by morp-hological observation,physiological and biochemical tests and 16S rRNA sequencing analysis.Under the initial condition,the maximum enzyme activity of filter paper was 84.25 U·L^(-1),and the degradation rate of filter paper was 20.21%on the 15 th day of culture.The optimal filter paper degradation condition was 0.2462 g·L^(-1) calcium chloride,27%liquid content,169 rpm,and the degradation rate of filter paper reached 33.25%.Compared with the optimization before 64.52%.Streptomyces microflavus CLSD-1 could be used as a potential microbial resource for degradation of low-temperature saline-alkali resistant cellulose.

关 键 词：低温 盐碱 纤维素降解 细黄链霉菌 

分 类 号：Q1[生物学—普通生物学]