大庆湿地沉积物中6株可培养蓝藻的分离与鉴定  

Isolation and Identification of Six Cultivable Cyanobacteria from Sediment Samples in Daqing City Wetland

作　　者：王鑫淼 姜雪 王丽艳[1] 李喜梅 吴楠 荆瑞勇[1,2] Wang Xinmiao;Jiang Xue;Wang Liyan;Li Ximei;Wu Nan;Jing Ruiyong(Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Argo-waste in Cold Region/College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319;Key Laboratory of Low-carbon Green Agriculture in Northeast Plain,Ministry of Agriculture and Rural Affairs)

机构地区：[1]黑龙江省寒区环境微生物与农业废弃物资源化利用重点实验室/黑龙江八一农垦大学生命科学技术学院,大庆163319 [2]农业农村部东北平原农业绿色低碳重点实验室

出　　处：《黑龙江八一农垦大学学报》2025年第1期105-111,126,共8页journal of heilongjiang bayi agricultural university

基　　金：国家重点研发计划(2023YFD2301601);国家自然科学基金面上项目(31870477);黑龙江省自然科学基金面上项目(C2017045)。

摘　　要：研究从大庆湿地6个沉积物样点中分离纯化了6株蓝藻,通过光学显微镜观察藻株的形态特征,并采用16S rRNA基因序列分析其系统发育关系,以明确大庆湿地沉积物中的可培养蓝藻种类。结果表明:所分离纯化的6株蓝藻中来自扎龙湿地的蓝藻ZLW-1隶属于盖丝藻属(Geitlerinema);来自连环湖的两株蓝藻LHH-2和LHH-3分别隶属于Ancylothrix属和微鞘藻属(Microcoleus);来自南引水库的NYS-5与席藻属(Phormidium)聚为一支,但其bootstrap值支持率为71%,很可能是未鉴定分类的新藻种,初步归属于Phormidium属;来自南引水库的NYS-3和NYS-4隶属于聚球藻属(Synechococcus)。综上,此结果丰富了大庆湿地为沉积物中可培养蓝藻的类群,为蓝藻的分类鉴定及菌种资源的开发奠定基础。Six cultivable cyanobacteria were isolated from six sediment samples in Daqing wetland.Their morphological characteristics were observed by optical microscope,and their phylogenetic relationship were analyzed based on 16S rRNA sequence for clarifying the cyanobacteria species in Daqing wetland.The results showed that ZLW-1 from Zhalong wetland belonged to Geitlerinema,LHH-2 and LHH-3 from Lake Lianhuan belonged to Ancylothrix and Microcoleus,respectively.NYS-5 from Nanyin reservoirs was grouped with Phormidium in phylogenetic tree,but bootstrap value was 71%.It was probably a new species of cyanobacteria that had not been identified.Therefore,NYS-5 belonged to Phormidium preliminarily.NYS-3 and NYS-4 from Nanyin reservoirs belonged to Synechococcus.This results enriched the resources of cultivable cyanobacteria in Daqing wetland sediments.It laid a foundation for the classification and identification of cyanobacteria and the development of microbial resources.

关 键 词：可培养蓝藻 沉积物 形态特征 系统发育分析 

分 类 号：Q938.1[生物学—微生物学]