重组PETase降解聚对苯二甲酸乙二醇酯  

Degradation of polyethylene terephthalate by recombinant PETase

作　　者：刘炫伶 方婕 林佳琪 王儒星 钟可尔 尹龙飞[1] LIU Xuanling;FANG Jie;LIN Jiaqi;WANG Ruxing;ZHONG Keer;YIN Longfei(Taizhou Key Laboratory of Biomass Functional Materials Development and Application,School of Life Sciences,Taizhou University,Taizhou 318000,China)

机构地区：[1]台州学院生命科学学院台州市生物质功能材料开发与应用重点实验室,浙江台州318000

出　　处：《化工科技》2025年第1期24-28,共5页Science & Technology in Chemical Industry

基　　金：国家级大学生创新创业训练计划项目(202310350028);台州市科技计划项目(22aqb03)。

摘　　要：聚对苯二甲酸乙二醇酯(PET)是一种被广泛应用于各个行业的合成聚酯,给人类生活带来便利的同时也造成了难以处理的土壤污染。利用生物信息学方法挖掘了来源于Schlegelella brevitaleapetase的基因,通过化学合成petase基因,再将其连接到基因表达载体pET-28b上并导入E.coli BL21,成功构建出高效表达PETase的重组基因工程菌BL21-pET28b-petase。进一步对PET降解活性进行测定,t=40℃,pH=7,ρ(酶)=30μg/mL,孵育时间为18 h, PETase降解PET的效果最佳,且表现较强的酶稳定性。为PET资源的循环利用和环境治理等绿色环保的处理奠定一定的实验基础。Polyethylene terephthalate is a kind of synthetic polyester which is widely used in various industries.It not only brings convenience to human life,but also causes difficult to deal with soil pollution.In this study,the petase gene derived from Schlegelella brevitaleapetase was mined by bioinformatics method and chemically synthesized,then connected to the gene expression vector pET-28b and the recombinant plasmid was transformed into E.coli BL21.Recombinant genetically engineered bacterium BL21-pET28B-petase that efficiently expressed PETase was successfully constructed.The degradation activity of PETase was determined.When the temperature was 40℃,the pH was 7,the enzyme concentration was 30μg/mL and the incubation time was 18 h,PETase had the best effect in degrading PET.The PETase exhibited strong stability.It lays a certain experimental foundation for providing a green treatment scheme for global issues such as the recycling of PET resources environmental management.

关 键 词：PET降解酶 聚对苯二甲酸乙二醇酯 PETase 生物降解 

分 类 号：X705[环境科学与工程—环境工程]