延伸因子Elongator Complex Protein 2抗油菜菌核病的功能研究  

Function of elongator complex protein 2 against Sclerotinia sclerotiorum of rapeseed

作　　者：何督 张岩 罗倩 傅玉全 杜雪竹[1] HE Du;ZHANG Yan;LUO Qian;FU Yu-quan;DU Xue-zhu(School of Life Sciences of Hubei University,Hubei Hongshan Laboratory,Wuhan 430062,China)

机构地区：[1]湖北大学生命科学学院,湖北省洪山实验室,湖北武汉430062

出　　处：《中国油料作物学报》2025年第1期60-70,共11页Chinese Journal of Oil Crop Sciences

基　　金：科技创新2030——重大项目(2023ZD04070);武汉市生物育种与新品种选育关键技术科技重大专项(2022021302024851);农业农村部油料作物生物学与遗传育种重点实验室开放课题基金(KF2022001)。

摘　　要：油菜生产受菌核病制约。为揭示甘蓝型油菜延伸因子复合体编码基因的抗病潜力,利用同源重组法构建了BnA06.ELP2基因的过表达载体和基因编辑的敲除载体,通过遗传转化获得了转基因株系。抗病相关试验显示,BnA06.ELP2过表达转基因植株相比野生型表现出更好的抗病性;而BnA06.ELP2的突变则会导致抗性降低。RT-PCR分析表明,BnA06.ELP2可调控茉莉酸/乙烯(JA/ET)通路标志基因过氧化氢酶BnCAT1和BnCAT2、茉莉酸合成相关基因BnLOX2和BnOPR1以及BnPDF1的转录。由此认为,油菜BnA06.ELP2参与油菜防御反应抵抗核盘菌侵染。Rapeseed production is severely restricted by Sclerotinia disease.Elongator complex protein(ELP)is a 6-subunit complex that interacts with RNA polymerase II,involved in plant defense response.To deeply understand its gene function,we constructed vectors on overexpressed-BnA06.ELP2 and knockout-BnA06.ELP2(by gene editing)using homologous recombination method,and then investigated their transgenic plants.Disease resistance experiments showed that BnA06.ELP2 overexpression plants exhibited better resistance compared to wildtype,while gene edited BnA06.ELP2 plants led to a decrease of resistance.Further RT-qPCR analysis indicated that BnA06.ELP2 could regulate transcription of catalase BnCAT1 and BnCAT2(marker genes of JA/ET pathway),jasmonate synthesis related genes BnLOX2 and BnOPR1,and BnPDF1.These results indicated that BnA06.ELP2 function in rapeseed is involved in defense response against Sclerotinia sclerotiorum infection.

关 键 词：甘蓝型油菜 延伸因子复合体 菌核病 

分 类 号：S565.4[农业科学—作物学]