二倍体野生种花生Oleosin基因家族全基因组鉴定及其表达分析  

Genome-wide analysis and stress-responsive expression profiling of the Oleosin gene family in diploid wild species Arachis duranensis and Arachis ipaensis

作　　者：姜焕焕 温思慧 卢雨庭 陈刚[1] 王通[2] JIANG Huan-huan;WEN Si-hui;LU Yu-ting;CHEN Gang;WANG Tong(School of Life Sciences,Zhaoqing University,Zhaoqing 526061,China;Shandong Peanut Research Institute,Qingdao 266100,China)

机构地区：[1]肇庆学院生命科学学院,广东肇庆526061 [2]山东省花生研究所,山东青岛266100

出　　处：《中国油料作物学报》2025年第1期94-104,共11页Chinese Journal of Oil Crop Sciences

基　　金：肇庆市科技创新指导项目(2022040316001);肇庆市科技局项目(2020N12003);肇庆学院校级项目(190060,QN202329);大学生创新创业训练计划项目(S202310580047,X202210580113)。

摘　　要：为鉴定花生油体蛋白(Oleosin)基因家族成员并分析其结构与功能,通过隐马尔可夫模型(Hiddne Markov Model,HMM)搜索二倍体野生花生Arachis duranensis和A. ipaensis蛋白质数据库,利用生物信息学在线软件对其理化性质、基因结构、基因定位进行分析预测,荧光定量PCR(quantitative real-time PCR, qRT-PCR)技术分析这些基因在花生不同组织器官及不同逆境处理条件下的表达模式。共获得7个AdOleosin基因和6个AiOleosin基因。除Aiole-2为酸性蛋白质外其他成员均为碱性蛋白。其结构以α-螺旋为主,存在2~3个跨膜区域,且有保守的中心疏水区和高度保守的脯氨酸结。AdOleosin和AiOleosin基因家族的染色体定位相似,在1和6号染色体没有定位,在4和7条染色体上各有3个家族成员。上游调控序列预测分析显示Oleosin基因存在大量的激素及逆境胁迫应答元件,PCR结果表明,Oleosin基因在花生根、茎、叶等组织中受干旱、盐及ABA胁迫诱导上调表达。且Oleosin基因在花生种子中表达量与温度有关。研究结果为深入研究Oleosin基因家族的生物学功能及花生抗逆品种的研究提供了支撑。To identify the members of Oleosin gene family and analyze its structure and function,Hiddne Markov Model(HMM)was used to search the protein databases of wild peanut(Arachis duranensis)and(Arachis ipaensis).The bioinformatic method was carried out to predict and analyse the physicochemical properties,gene structure,and gene location.The expression patterns of these genes in different peanut tissues and organs and under different stress conditions were analyzed by qRT-PCR.In this study a total of 7 AdOleosin genes and 6 AiOleosin genes were identified.Results showed that all Oleosin genes except Aiole-2 are acidic proteins.Further analysis showed that Oleosins were basic small molecular proteins withα-spiral as the main structure.They have 2-3 transmembrane domains,a conserved central hydrophobic region and a highly conserved proline junction.The AdOleosin and AiOleosin gene families have similar chromosomal localization,which no localization on chromosomes 1 and 6,and 3 family members each on chromosomes 4 and 7.There are multiple hormone and stress-related cis-acting elements,indicating that the gene family is involved in stress response.Further,the expression patterns in different tissues were investigated by qRT-PCR.Results showed that the Oleosin gene expressed in peanut seeds is temperature dependent and expression in peanut roots,stems,leaves were induced by drought stress,salt and ABA.The above results provide a theoretical basis for in-depth study of the gene function of the Oleosin family.

关 键 词：花生 Oleosin基因家族 生物信息学 表达分析 

分 类 号：S565.2[农业科学—作物学]