大豆根瘤内生菌合成菌群对根系微生物群落的影响  

Synthetic flora of soybean endophytes alter root microbial community structure and function

作　　者：汪芳芳 孙秀娟 徐伟慧[1] 陈文晶 胡云龙[1] 王志刚[1] WANG Fang-fang;SUN Xiu-juan;XU Wei-hui;CHEN Wen-jing;HU Yun-long;WANG Zhi-gang(College of Life Science and Agroforestry,Qiqihar University/Heilongjiang Provincial Technology Innovation Center of Agromicrobial Preparation Industrialization/Heilongjiang Provincial Collaborative Innovation Center of Agrobiological Preparation Industrialization,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院/黑龙江省农业微生物制剂产业化技术创新中心/黑龙江省农用生物制剂产业化协同创新中心,黑龙江齐齐哈尔161006

出　　处：《中国油料作物学报》2025年第1期192-203,共12页Chinese Journal of Oil Crop Sciences

基　　金：黑龙江省杰出青年基金(JQ2023D001);黑龙江省重点研发计划(GA21B007);黑龙江省高校基本科研业务费项目(145209805;145209810)。

摘　　要：为了解根瘤内生菌合成菌群对大豆生长和对根系微生物的影响,利用前期分离自大豆根瘤的根瘤内生菌,通过菌株功能的互补、多样性的增加和促生效果的叠加构建了4个合成菌群。本试验研究发现,菌群Q1和Q4促生效果最佳,菌群Q1可显著增加大豆植株的茎长、茎鲜重、根鲜重和根瘤数,分别提升了19.2%、44.0%、51.7%和140.0%;菌群Q4显著增加了大豆植株的总根长、根表面积、根鲜重和根瘤数,分别提升了24.3%、34.0%、40.0%和200.0%。根内的16S rRNA测序结果表明,根瘤内生菌中Bacillus和Klebsiella能够侵入大豆根内,使细菌群落间正相关关系和生态功能相关编码基因丰度增加。根际土壤的宏基因组测序结果表明,接种合成菌群后根际中富集了Nocardioides、Pseudonocardia等有益菌属,显著增加了参与氮代谢和吲哚-3-乙酸(IAA)生物合成途径相关基因的丰度,并促进根际细菌进行辅酶、氨基酸转运和代谢,碳水化合物代谢和信号转导等生物过程。本项研究结果为构建大豆内生微生物组及发展高效微生物肥料提供了理论依据和技术支持。To investigate the impact of synthetic bacterial flora of nodule endophytes on soybean growth and root microorganisms,this experiment used nodule endophytes isolated from soybean root nodules to construct four synthetic bacterial flora by complementation of strain functions,increase in diversity and superposition of growthpromoting effects.In the study,the bacterial groups Q1 and Q4 exhibited the best growth-promoting effects.Q1 significantly enhanced the stem length,stem fresh weight,root fresh weight and nodule number of soybean plants,increasing by 19.2%,44.0%,51.7%and 140.0%respectively.Q4 significantly increased the total root length,root surface area,root fresh weight and number of nodules of soybean plants,increasing by 24.3%,34.0%,40.0%and 200.0%respectively.The 16S rRNA sequencing results in the roots revealed that Bacillus and Klebsiella among the root nodule endophytes invaded soybean roots,increased the positive correlation among bacterial communities and the abundance of genes encoding genes related to ecological functions.Metagenomic sequencing results of rhizosphere soil showed that after inoculation with synthetic bacterial flora,beneficial bacterial genera such as Nocardioides and Pseudonocardia were enriched in the rhizosphere,and genes involved in nitrogen metabolism and indole-3-acetic acid(IAA)biosynthetic pathways were significantly increased,and rhizosphere bacteria to carry out biological processes such as coenzymes,amino acid transport and metabolism,carbohydrate metabolism and signal transduction were promoted.The results provided theoretical basis and technical support for the construction of soybean endophytic microbiome and the development of efficient microbial fertilizer.

关 键 词：大豆 根瘤内生菌 合成菌群 促生机制 微生物肥料 

分 类 号：S565.1[农业科学—作物学]