夏栎抗性基因MBF1家族生物信息学分析及逆境响应研究  

Bioinformatics Analysis and Stresses Response of QrMBF1 Family in Quercus robur L.

作　　者：王寒葶 张秋月 叶艺 吴涵 吴泓毅 陈小红[1] 杨汉波 惠文凯 WANG Hanting;ZHANG Qiuyue;YE Yi;WU Han;WU Hongyi;CHEN Xiaohong;YANG Hanbo;HUI Wenkai(College of Forestry,Sichuan Agricultural University/Sichuan Province Key Laboratory of Ecological Forestry Engineering on the Upper Reaches of the Yangtze River,Chengdu 611130,China)

机构地区：[1]四川农业大学林学院/四川省长江上游林业生态工程重点实验室,成都611130

出　　处：《四川农业大学学报》2025年第1期193-204,共12页Journal of Sichuan Agricultural University

基　　金：2023年大学生创新训练计划项目(S202310626074);四川省重点研发计划项目(2024YFNH0028);四川省自然科学基金面上项目(2024NSFSC0411);国家自然科学基金(32301614)。

摘　　要：【目的】探究夏栎抗性基因QrMBF1家族基因特征和逆境调控机制,为培育优质的夏栎种质资源提供理论基础及数据支持。【方法】结合已公布的夏栎基因组数据库,采用生物信息学研究方法对夏栎MBF1家族成员进行核酸和蛋白序列分析;采用荧光定量PCR技术解析夏栎QrMBF1s在7种不同组织中的表达模式及其对逆境的响应情况。【结果】夏栎共有2个MBF1家族成员,分别为429 bp位于8号染色体的QrMBF1b和432 bp位于2号染色体的QrMBF1c,且均为亲水性的稳定蛋白;QrMBF1家族成员的启动子序列均富含响应光照、低氧和干旱等非生物胁迫的顺式作用元件;QrMBF1b主要在根部高表达,而QrMBF1c在成熟茎、成熟叶和顶芽中表达量相对较高;夏栎QrMBF1s能够响应外源高温和水淹处理,其中QrMBF1c在逆境处理后3 h即可快速响应并显著上调。【结论】夏栎QrMBF1家族可能参与了植物的逆境调控过程,尤其是对水淹和高温条件的响应过程。本研究所获结果,对于进一步揭示QrMBF1s抗逆性的调控机制,推动夏栎抗性育种工作具有重要意义。【Objective】The study aimed to investigate the characteristics of the QrMBF1 gene family and its stress regulation mechanism in Quercus robur,thereby providing a theoretical foundation and data support for the cultivation of high-quality Q.robur germplasm resources.【Method】Utilizing the publicly available Q.robur genomic database,bioinformatics research methods were employed to analyze the nucleic acid and protein sequences of MBF1 family members in Q.robur.Quantitative real-time PCR technology was used to examine the expression patterns of QrMBF1s in seven different tissues and under various stress conditions.【Result】The Q.robur MBF1 family comprises two members:QrMBF1b located on chromosome 8 with a length of 429bp and QrMBF1c located on chromosome 2 with a length of 432 bp.Both proteins are hydrophilic and stable.The promoter sequences of QrMBF1 family members are enriched with transcription factor binding sites and cis-acting elements related to stress resistance regulation,such as ERF and LBD.QrMBF1b is predominantly expressed in roots,whereas QrMBF1c exhitits relatively higher expression levels in mature stems,mature leaves,and apical buds.QrMBF1s in Q.robur respond to exogenous high temperature and waterlogging treatments,with QrMBF1c showing a rapid and significantly upregulated after 3 hours of stress treatment.【Conclusion】The QrMBF1 family may a role in plant's adversity regulation processes,especially in responses to flooding and high temperature conditions.These findings are significant for elucidating the regulatory mechanism of QrMBF1s in stress resistance and advancing the breeding of Q.robur.

关 键 词：夏栎 MBF1 植物抗逆 生物信息学分析 

分 类 号：S718.3[农业科学—林学]