紫花苜蓿MsSAUR33通过ABA信号转导途径负调控叶片衰老  

Alfalfa MsSAUR33 Negatively Regulates Leaf Senescence via ABA Signal Transduction

作　　者：刘春丽 郑川 李孟湛 尹红菊 LIU Chunli;ZHENG Chuan;LI Mengzhan;YIN Hongju(College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China)

机构地区：[1]兰州大学草地农业科技学院,兰州730020

出　　处：《四川农业大学学报》2025年第1期205-214,共10页Journal of Sichuan Agricultural University

基　　金：甘肃省政府国资委关键核心技术攻关项目(2023GE008);国家资助博士后研究人员计划(GZB20230274)。

摘　　要：【目的】研究MsSAUR33在植物叶片衰老过程中的功能。【方法】构建紫花苜蓿MsSUR33超表达植物载体,将其超表达于野生型拟南芥与紫花苜蓿中,并测定叶片衰老相关指标。【结果】随着叶片衰老,MsSAUR33下调表达。MsSAUR33超表达拟南芥和紫花苜蓿株系的叶绿素含量均显著高于野生型。进一步研究发现,脱落酸(ABA)处理导致MsSAUR33下调表达,且MsSAUR33能与ABA信号转导途径中的蛋白激酶MsSnRK2.3相互作用。【结论】MsSAUR33通过介导ABA信号转导途径在叶片衰老中发挥负调控作用。【Objective】This study is to investigate the function of MsSAUR33 during plant leaf senescence.【Method】An over-expression vector of MsSAUR33 from alfalfa was constructed and transformed into wild-type Arabidopsis thaliana(Arabidopsis)and alfalfa to measure the related indexes of leaf senescence.【Result】The expression of MsSAUR33 was found to be down-regulated during leaf senescence.Arabidopsis and alfalfa lines overexpressing MsSAUR33 exhibited significantly higher chlorophyll content compared to their wild-type plants.Further studies revealed that abscisic acid(ABA)treatment resulted in down-regulation of MsSAUR33,and MsSAUR33 was shown to interact with MsSnRK2.3,a protein kinase involved in ABA signal transduction.【Conclusion】MsSAUR33 plays a negative regulatory role in leaf senescence by mediating the ABA signal transduction.

关 键 词：紫花苜蓿 MsSAUR33 叶片衰老 脱落酸(ABA) 

分 类 号：S541.9[农业科学—作物学]