春尺蠖AcinTre2基因的鉴定、时空与逆境胁迫下的表达  

Molecular Characteristics,Spatiotemporal and Adversity Stress Expression Analysis of Trehalase Gene AcinTre2 in Apocheim acinerarius(Lepidoptera:Geometridae)

作　　者：陈龙 李东伟 朱圣杰 郝水源 赵嘉庆 崔阔澍 CHEN Long;LI Dongwei;ZHU Shengjie;HAO Shuiyuan;ZHAO Jiaqing;CUI Kuoshu(Agriculture Department,Hetao College,Bayannur 015000,Inner Mongolia,China;Hetao Area Green Agricultural Product Safety Production and Early Warning Control Laboratory,Bayannur 015000,Inner Mongolia,China;Bayannur Graziery Service Center,Bayannur 015000,Inner Mongolia,China;Sichuan Agricultural Technology Extension Station,Chengdu 610041,China)

机构地区：[1]河套学院农学系,内蒙古巴彦淖尔015000 [2]河套地区绿色农产品安全生产与预警控制实验室,内蒙古巴彦淖尔015000 [3]巴彦淖尔市畜牧业服务中心,内蒙古巴彦淖尔015000 [4]四川省农业技术推广总站,成都610041

出　　处：《四川农业大学学报》2025年第1期215-223,共9页Journal of Sichuan Agricultural University

基　　金：内蒙古自然科学基金(2021BS03028);“科技兴蒙”行动重点专项(NMKJXM202201);四川省中央“三区”科技人才支持计划。

摘　　要：【目的】旨在明确春尺蠖海藻糖酶基因AcinTre2的分子特征、系统进化及时空与逆境胁迫下的表达情况,进而探究该基因在春尺蠖生长发育及在逆境胁迫中的作用。【方法】基于前期所测春尺蠖蛹转录组数据,获取AcinTre2基因序列信息,采用qPCR技术检测AcinTre2基因在不同发育阶段、组织与逆境胁迫下的表达模式。【结果】春尺蠖AcinTre2基因完整CDS序列全长为1920 bp,编码639个氨基酸,蛋白质预测分子量为73.89 kDa,预测等电点为5.89。系统进化结果表明,AcinTre2与棉铃虫Helicoverpa armigera、甜菜夜蛾Spodoptera exigua和斜纹夜蛾Spodoptera litura的膜结合蛋白基因聚为一类。基因表达结果显示,AcinTre2在春尺蠖整个发育阶段均有表达但处于较低水平,在雌虫中表达量最高,且与其他阶段有显著差异(P<0.05);在春尺蠖雌虫腹中表达量最高,其次为雄虫的足。雌虫腹中的表达量极显著高于雄虫,而在雄虫头和胸部中的表达量则极显著高于雌虫;在春尺蠖3龄幼虫4℃处理3 h后,表达量达到最大值;随着饥饿处理时间的增加,AcinTre2在0~24 h间表达量没有显著差异,48 h后出现下降,在饥饿处理72 h后达到最大值;随着温度上升,表达量呈现先上升后下降的趋势,在0℃下处于最大值,且与其余温度的表达量差异显著(P<0.05)。【结论】AcinTre2基因与春尺蠖生长发育和在抵御逆境胁迫中的响应有密切的关系。该结果为进一步探究该基因在春尺蠖生长发育与逆境胁迫中的分子功能奠定一定理论基础。【Objective】This study aims to elucidate the molecular characteristics,phylogenetic evolution,and spatiotemporal expression patterns of the AcinTre2 trehalase gene in Apocheim acinerarius under stress conditions.The objective is to investigate the gene's role in the growth,development,and stress response of A.acinerarius.【Method】Using previously sequenced pupal transcriptome data from A.acinerarius,we obtained the AcinTre2 gene sequence information.Quantitative qPCR technology was employed to analyze the expression patterns of the AcinTre2 gene at various developmental stages,tissues,and under different stress conditions.【Result】The complete CDS sequence of the AcinTre2 gene in A.acinerarius is 1920 bp,encoding a protein of 639 amino acids with a predicted protein molecular weight of 73.89 kDa and an isoelectric point of 5.89.Phylogenetic analysis revealed that AcinTre2 is closely related to membrane-bound protein genes in species such as Heli⁃coverpa armigera,Spodoptera exigua and Spodoptera litura.The AcinTre2 gene is expressed throughout the development of the A.acinerarius,with the highest expression observed in females,which is significantly different from other stages(P<0.05).In females,the abdomen exhibits the highest expression,followed by the legs of males.The expression in the female abdomen is significantly higher than in males,whereas in males,the expression in the head and thorax is significantly higher than in females.After a 3-hour treatment at 4℃in third instar larvae,the expression reaches its maximum.The expression remains unchanged during the first 24 hours of starvation,decreases after 48 hours,and reaches a maximum after 72 hours of starvation.As temperature increases,the expression pattern shows an initial increase followed by a decrease,with the maximum expression observed at 0℃,which is significantly different from other temperatures(P<0.05).【Conclusion】The AcinTre2 gene is closely associated with the growth,development,and stress response of the A.acinerarius.These fi

关 键 词：春尺蠖 AcinTre2 低温胁迫 饥饿胁迫 表达谱 

分 类 号：S888.721[农业科学—特种经济动物饲养]