基于BSA-seq技术定位番茄花序分枝基因  

Mapping of Inflorescence Branching Genes in Tomato Based on BSA-seq

作　　者：孔济升 刘志强 马静 李仁 崔霞 孙帅[2] KONG Jisheng;LIU Zhiqiang;MA Jing;LI Ren;CUI Xia;SUN Shuai(College of Horticulture,Qingdao Agricultural University,Qingdao 266109,Shandong,China;State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]青岛农业大学园艺学院,山东青岛266109 [2]中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京100081

出　　处：《中国蔬菜》2025年第2期56-63,共8页China Vegetables

基　　金：国家自然科学基金重点国际合作研究项目(32120103010);北京市种质创制和品种选育联合攻关项目(G20220628003-03);国家自然科学基金青年科学基金项目(32102403)。

摘　　要：花序分枝数量决定番茄果实数量和大小,是番茄产量形成的重要因素,也是番茄育种的重要目标性状。为了定位并克隆花序分枝关键调控基因,以复杂花序分枝的ST198和简单花序分枝的ST211为亲本,构建F2定位群体。通过混池重测序技术,发现了5个调控花序分枝的主效QTL。对主效QTL(qIB12.2)进行分析和验证发现,J2为调控花序分枝的关键基因,并发现J2对另一主效QTL位点(qIB12.4)具有上位性效应。同时将qIB12.4进行精细定位于446.7 kb区间,并开发了紧密连锁的分子标记。研究丰富和完善了番茄花序分枝形成的遗传基础和调控网络,对番茄花序分枝发育机理的解析及精准分子育种具有重要理论意义和应用价值。Inflorescence-branching structure determines the number and size of tomato fruit,which is an important component of tomato yield and a main objective in tomato breeding.To identify and clone the key regulatory genes involved in inflorescence branching,‘ST198’with complex inflorescence branching and‘ST211’with simple inflorescence branching were selected as parental lines to generate an F2 mapping population.Five major quantitative trait locis(QTLs)regulating inflorescence branching were identified using BSA-seq.Analysis and validation of the major QTL,qIB12.2,revealed that J2 was the key gene regulating inflorescence branching.J2 was found to exhibit an epistatic effect on another major QTL,qIB12.4.What’s more,qIB12.4 was finely mapped to the 446.7 kb region,and closely linked molecular markers were developed.Our findings provide insights into the genetic regulatory network underlying tomato inflorescence development and have important application value for precise molecular breeding of tomato.

关 键 词：番茄 花序形态 QTL 产量性状 

分 类 号：S641.2[农业科学—蔬菜学]